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Agrisera
GID1c/Gibberellin receptor GID1C Polyclonal Antibody
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GID1c/Gibberellin receptor GID1C Antibody (AS14-2800) in WB
20 µg of total protein from Arabidopsis thaliana cauline leaves (C. L. ), seedling (S. L. ) material or imbibed seeds (I. S. ) was extracted in 50 m M Phosphate buffer (p H 7.0) with 1 X protease inhibitor (Sigma Aldrich) and was separated on a TGX and KD SDS-PAGE gel (Bio-Rad) and blotted 14 min to PVDF using Bio-Rad semi-dry turbo transfer system. Blots were blocked with 2% ECL advance (GE healthcare) for... View More
产品信息
AS14-2800
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant full-length Arabidopsis thaliana GID1c.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
GID1C encodes a soluble gibberellin (GA) receptor that functions predominantly in the nucleus (with evidence that GID1 family receptors can also be detected in the cytosol) to couple GA perception to transcriptional reprogramming via DELLA protein removal. The GID1C protein is a single-polypeptide, alpha/beta-hydrolase fold member with sequence similarity to hormone-sensitive lipase/carboxylesterase-like proteins, retaining conserved hydrolase-family motifs (including HGG and GXSXG) but lacking key features required for canonical lipase catalysis, consistent with a ligand-binding/signaling role rather than enzymatic hydrolysis. Structurally, GA binding occurs in a deep pocket and induces conformational changes that promote high-specificity recognition of the N-terminal DELLA domain motifs of DELLA repressors, enabling formation of a GA-GID1-DELLA complex that triggers DELLA ubiquitination and proteasome-dependent degradation, thereby releasing repression of GA-responsive growth and developmental programs. Biologically, Arabidopsis GID1 receptors show partial redundancy, but GID1C contributes distinct, tissue-context functions; in reproductive development, GID1C expression is enriched in fruit valves and genetic analyses support roles in GA-dependent pod elongation/fruit growth through specific receptor-DELLA pairings in those tissues.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT5G27320.1; ATGID1C; F21A20.30; F21A20_30; GA INSENSITIVE DWARF1C
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