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Invitrogen
GL7 Monoclonal Antibody (GL-7 (GL7)), eFluor™ 450, eBioscience™
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GL7 Antibody (48-5902-82) in Flow
Staining of unstimulated (left) or 3-day Con A-stimulated (right) C57Bl/6 splenocytes with Anti-Mouse CD3 PerCP-eFluor® 710 (Product # 46-0032-82) 0.25 µg of Anti-Human/Mouse GL7 (T and B Cell Activation Marker) eFluor® 450. Total viable cells were used for analysis.
产品信息
48-5902-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
C. elegans,
Human,
Mouse
宿主/亚型
Rat
/ IgM
分类
Monoclonal
类型
Antibody
克隆号
GL-7 (GL7)
偶联物
激发/发射光谱
405/445 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.
Applications Reported: This GL-7 (GL7) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This GL-7 (GL7) antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
GL7 is a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
