Invitrogen

GLUT1 Recombinant Rabbit Monoclonal Antibody (SA0377)

Name: GLUT1 Recombinant Rabbit Monoclonal Antibody (SA0377)
Brand: Invitrogen
SKU: MA5-31960

高级验证

2个已发表图片

8 篇参考文献

查看其他28个GLUT1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

GLUT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GLUT1 Antibody (MA5-31960) in IHC (P)

Immunohistochemical analysis of GLUT1 of paraffin-embedded Human breast carcinoma tissue using a GLUT1 Monoclonal antibody (Product #MA5-31960). Counter stained with hematoxylin.

GLUT1 Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

GLUT1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with GLUT1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-GLUT1 Recombinant Rabbit Monoclonal Antibody (SA0377) (Product # MA5-31960). GLUT-1 shows a streak like pattern in cell lines as seen in all the 3 lanes. The siRNA Lane shows almost 90% loss of streak like signal. {KD}

Immunogen image

Immunogen Image GLUT1 Recombinant Rabbit Monoclonal Antibody (SA0377)

产品信息

MA5-31960

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:50,000-1:100,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:5,000-1:10,000

免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

1:500-1:1,000

流式细胞分析 (Flow)

1:500-1:1,000

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SA0377

抗原

Synthetic peptide within Human GLUT1 aa 443-492

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.115 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2809254

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Glucose transporter 1 (GLUT1), encoded by the SLC2A1 gene, is a facilitative glucose transporter that plays a pivotal role in the uptake of glucose across cell membranes, particularly in tissues with high-energy demands such as the brain, erythrocytes, and endothelial cells of the blood-brain barrier. GLUT1 is ubiquitously expressed and functions through a passive diffusion mechanism, allowing glucose to move down its concentration gradient. Its involvement in maintaining glucose homeostasis is crucial for cellular metabolism and energy production. Mutations in SLC2A1 can lead to GLUT1 deficiency syndrome, characterized by seizures, developmental delay, and movement disorders due to impaired glucose transport into the brain. GLUT1 also plays a significant role in cancer biology, where its overexpression is commonly observed in various tumors, facilitating the enhanced glucose uptake required for rapid tumor cell proliferation. This characteristic makes GLUT1 a potential target for cancer diagnostics and therapeutics. Additionally, GLUT1's regulation is influenced by factors such as hypoxia, insulin, and cellular stress, highlighting its adaptive role in different physiological and pathological states.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity); glucose transporter glut1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; GT1; HepG2 glucose transporter; human T-cell leukemia virus (I and II) receptor; MGC141895; MGC141896; receptor for HTLV-1 and HTLV-2; solute carrier family 2 (facilitated glucose transporter), member 1; Solute carrier family 2 a 1 (facilitated glucose transporter) brain; Solute carrier family 2, facilitated glucose transporter member 1; solute carrier family 2, member 1

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基因别名: CSE; DYT17; DYT18; DYT9; EIG12; GLUT; GLUT-1; GLUT1; GLUT1DS; GLUTB; GTG1; Gtg3; HTLVR; PED; RATGTG1; SDCHCN; SLC2A1

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UniProt ID: (Human) P11166, (Mouse) P17809, (Rat) P11167

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Entrez Gene ID: (Human) 6513, (Mouse) 20525, (Rat) 24778

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功能