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Invitrogen
GLUT4 Monoclonal Antibody (3G10A3)
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GLUT4 Antibody (MA5-17176) in ICC/IF
Immunofluorescence analysis of GLUT4 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GLUT4 Monoclonal Antibody (3G10A3) (Product # MA5-17176, 1:200) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32766, 1:... View More
产品信息
MA5-17176
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
C. elegans,
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
3G10A3
抗原
Purified recombinant fragment of human SLC2A4 (amino acids: 224-353 ) expressed in E. Coli.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA5-17176 targets SLC2A4 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Mouse samples.
The MA5-17176 immunogen is purified recombinant fragment of human SLC2A4 (amino acids: 224-353 ) expressed in E. Coli.
MA5-17176 detects SLC2A4 which has a predicted molecular weight of approximately 54.8kDa.
靶标信息
This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: glucose transporter glut4; Glucose transporter type 4, insulin-responsive; GLUT-4; GT2; insulin-responsive glucose transporter type 4; MGC93607; solute carrier family 2 (facilitated glucose transporter), member 4; Solute carrier family 2, facilitated glucose transporter member 4
基因别名: Glut-4; GLUT4; SLC2A4
UniProt ID: (Human) P14672, (Mouse) P14142
Entrez Gene ID: (Human) 6517, (Mouse) 20528
carbohydrate metabolic process
amylopectin biosynthetic process
glucose transport
cellular response to insulin stimulus
glucose homeostasis
response to ethanol
glucose import
brown fat cell differentiation
cellular response to hypoxia
cellular response to osmotic stress
glucose transmembrane transport
transport
carbohydrate transport
proton transport
hexose transmembrane transport
transmembrane transport
cation transmembrane transport
