Invitrogen

GOLPH3 Polyclonal Antibody

Name: GOLPH3 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-95558

查看其他15个GOLPH3抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (5)

GOLPH3 Antibody in Immunocytochemistry (ICC/IF)

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GOLPH3 Antibody (PA5-95558) in ICC/IF

Immunocytochemistry analysis of GOLPH3 using anti-GOLPH3 antibody (Product # PA5-95558) . GOLPH3 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5 μg/mL rabbit anti-GOLPH3 antibody (Product # PA5-95558) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was co... View More

GOLPH3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen Image GOLPH3 Polyclonal Antibody

产品信息

PA5-95558

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human GOLPH3 recombinant protein (Position: K56-K298).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2807360

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human 293T whole cell, human MDA-MB-453 whole cell, human A431 whole cell, rat lung tissue, rat testis tissue, mouse lung tissue, mouse testis tissue. IHC: human intestinal cancer tissue, mouse brain tissue, rat brain tissue. ICC/IF: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signaling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signaling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Coat protein GPP34; coat-protein; golgi peripheral membrane protein 1, 34 kDa; Golgi phosphoprotein 3; golgi phosphoprotein 3 (coat-protein); golgi protein; golgi-associated protein; MIDAS; Mitochondrial DNA absence factor; trans-Golgi protein GMx33

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基因别名: 4733401N08Rik; 5730410D03Rik; AW413496; Gmx33; GOLPH3; GOPP1; GPP34; MIDAS; Vps74

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UniProt ID: (Human) Q9H4A6, (Mouse) Q9CRA5

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Entrez Gene ID: (Human) 64083, (Mouse) 66629, (Rat) 78961

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功能

protein binding enzyme binding phosphatidylinositol-4-phosphate binding lipid binding

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