Invitrogen

GRK1 Monoclonal Antibody (G8)

Name: GRK1 Monoclonal Antibody (G8)
Brand: Invitrogen
SKU: MA1-720

高级验证

15个已发表图片

23 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

GRK1 Antibody in Immunocytochemistry (ICC/IF)

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GRK1 Antibody (MA1-720) in ICC/IF

Immunofluorescence analysis of Rhodopsin Kinase 1a was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rhodopsin Kinase 1a (G8) Mouse Monoclonal Antibody (Product # MA1-720) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjuga... View More

GRK1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

GRK1 Antibody — Figure 2 Protein expression in wild-type, Arr1 +/- , GRK1 +/- , and GRK1 +/- Arr1 +/- rods. (A) Representative immublot analysis of arrestin and GRK1 expression in retinas of wild-type and heterozygous knockout mice. The blot was probed with polyclonal anti-arrestin, monoclonal anti-rhododopsin kinase 1a, and monoclonal anti- beta tubulin antibodies. Two different dilutions of the same samples were run on the same blot. (B) Levels of arrestin1 and GRK1 expression in heterozygous knockout retinas normalized to the levels in wild-type retinas. Integrated fluorescence intensity values were used for all analyses. Variations in sample loading were corrected for by normalizing the intensity value of each protein band to the intensity value of beta -tubulin in the same lane. Open circles and triangles show the results from two different dilutions. Normalized ratios of arrestin1 and GRK1 expression compared to wild-type values for Arr1 +/- were 0.34 +- 0.06 and 0.87 +- 0.07 (mean +- SEM, n = 5); 1.14 +- 0.10 and 0.32 +- 0.03 for GRK1 +/- (n = 4); and 0.54 +- 0.09 and 0.34 +- 0.03 for GRK1 +/- Arr1 +/- (n = 5). (C) Levels of PDE alphabeta , PDE gamma and RGS9-1 expression in heterozygous retinas normalized to the levels in wild-type retinas. Normalized ratios of PDE alphabeta , PDE gamma and RGS9-1 compared to wild-type values for Arr1 +/- were 0.97 +- 0.03, 1.06 +- 0.05, and 1.05 +- 0.07 (n = 4); 0.86 +- 0.03, 0.99 +- 0.04, and 1.05 +- 0.07 for GRK1 +/- (n = 4); 0.89 +- 0.05, 0.99 +- {KO}

Immunogen image

Immunogen Image GRK1 Monoclonal Antibody (G8)

产品信息

MA1-720

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫组化 (IHC)

Assay-dependent

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免疫细胞化学 (ICC/IF)

2-3 µg/mL

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免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Bovine, Chicken, Human, Mouse, Rat

已发表种属

Bovine, Dog, Human, Mouse, Rat, Zebrafish

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

抗原

Full length human GRK1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2115613

产品详细信息

MA1-720 detects G-protein-associated rhodopsin kinase 1a (GRK1a) from human, mouse, rat, bovine and chicken tissues.

MA1-720 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~60 kDa protein representing GRK1a from bovine rod outer segments. Immunofluorescence staining of GRK1a in bovine retina with MA1-720 results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods.

The MA1-720 immunogen is full length human GRK1. This antibody recognizes an epitope in the C-terminal region of GRK1a.

靶标信息

GRK1 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinases. GRK1 mediates rapid desensitization of rod photoreceptors to light by catalyzing phosphorylation of the visual pigment rhodopsin which leads to its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). GRK1 can be phosphorylated by PKA and this reduces the ability of GRK1 to phosphorylate rhodopsin in vitro. It is speculated that phosphorylation of GRK1 by PKA in vivo occurs in the dark when cAMP levels in photoreceptor cells are elevated.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: G protein-coupled receptor kinase 1; G protein-coupled receptpr kinase 1; G-protein receptor kinase 1; GRK 1a/b; GRK1a; rhodopsin kinase; Rhodopsin kinase GRK1; RK

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基因别名: GPRK1; GRK1; RHOK; RK

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UniProt ID: (Human) Q15835, (Bovine) P28327, (Rat) Q63651