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Invitrogen
GSDMD (Cleaved Gly276) Recombinant Rabbit Monoclonal Antibody (2S1G2)
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GSDMD (Cleaved Gly276) Antibody (MA569594) in WB
Western blot analysis of GSDMD (Cleaved Gly276) in THP-1 cells. Samples were probed with GSDMD (Cleaved Gly276) recombinant monoclonal antibody (Product # MA569594) at 1:1,000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3 % nonfat dry milk in TBST.
产品信息
MA569594
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
2S1G2
抗原
Synthetic peptide.
偶联物
形式
Liquid
浓度
0.95 mg/mL
规格
95 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3., with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
靶标信息
GSDMD (Cleaved Gly276) refers to the activated form of gasdermin D generated following proteolytic cleavage at glycine 276, a key step in the execution of pyroptosis. Gasdermin D is encoded by the GSDMD gene and is synthesized as a full-length protein composed of an N-terminal pore-forming domain and a C-terminal autoinhibitory domain. Upon activation of inflammatory caspases-most notably caspase-1 in canonical inflammasome pathways and caspase-4/5 (human) or caspase-11 (mouse) in noncanonical pathways-GSDMD is cleaved at a specific linker region near Gly276. This cleavage releases the N-terminal fragment, which oligomerizes and inserts into the plasma membrane to form pores, leading to cell swelling, membrane rupture, and the release of pro-inflammatory cytokines such as IL-1β and IL-18. Detection of cleaved GSDMD (Gly276) is widely used as a molecular marker of inflammasome activation and pyroptotic cell death in inflammation and innate immunity research.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: breast cancer related protein; gasdermin domain containing 1; Gasdermin domain-containing protein 1; Gasdermin-D; unnamed protein product
基因别名: DF5L; DFNA5L; FKSG10; GSDMD; GSDMDC1
UniProt ID: (Human) P57764
Entrez Gene ID: (Human) 79792
plasma membrane repair
immune system process
inflammatory response
protein secretion
programmed cell death
positive regulation of interleukin-1 beta production
positive regulation of interleukin-1 production
positive regulation of interleukin-18 production
interleukin-18-mediated signaling pathway
defense response to bacterium
NLRP3 inflammasome complex assembly
innate immune response
ceramide biosynthetic process
pore complex assembly
positive regulation of inflammatory response
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
protein homooligomerization
protein maturation
transmembrane transport
pyroptosis
interleukin-1-mediated signaling pathway
pyroptotic cell death
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