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Invitrogen
GTF2A1 Polyclonal Antibody
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GTF2A1 Antibody (PA5-102739) in ICC/IF
Immunofluorescent analysis of GTF2A1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and GTF2A1 polyclonal antibody (Product # PA5-102739) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-102739
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human GTF2A1(Accession P52655), corresponding to amino acid residues D284-V334.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total GTF2A1.
靶标信息
Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A) and several general initiation factors. One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: general transcription factor 2a, 1; general transcription factor IIA 1; General transcription factor IIA subunit 1; general transcription factor IIa, 1 (37kD and 19kD subunits); general transcription factor IIA, 1, 19/37kDa; glucose regulated protein, 58kD pseudogene; TFIIA alpha, p55, isoform 1; TFIIA alpha/beta; TFIIA alpha/beta subunits; TFIIA large subunit; TFIIA-42; TFIIAL; Transcription initiation factor IIA subunit 1; Transcription initiation factor TFIIA 42 kDa subunit
基因别名: 6330549H03Rik; AA536742; AA959775; AW060250; GTF2A1; Tf2a; TF2A1; TFIIA; TFIIA-42; Tfiia1; TfIIAa/b; TFIIAL
UniProt ID: (Human) P52655, (Mouse) Q99PM3, (Rat) O08949
Entrez Gene ID: (Human) 2957, (Mouse) 83602, (Rat) 83830
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
snRNA transcription from RNA polymerase II promoter
transcription, DNA-templated
biological_process
