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Invitrogen
GTF2E1 Recombinant Rabbit Monoclonal Antibody (23GB6310)
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GTF2E1 Antibody (MA5-53018) in WB
Western Blot analysis of GTF2E1. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GTF2E1 recombinant monoclonal antibody (Product # MA5-53018) with a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit.
产品信息
MA5-53018
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293F
分类
Recombinant Monoclonal
类型
Antibody
克隆号
23GB6310
抗原
A synthesized peptide derived from human TFIIE alpha (400-439AA).
偶联物
形式
Liquid
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57, 000 (TFIIE-alpha) and 34, 000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF. TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay. Its sequence contains several interesting structural motifs (leucine repeat, zinc finger and helix-turn-helix) and sequence similarities to bacterial sigma factors that suggest direct involvement in the regulation of transcription initiation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: general transcription factor II E, polypeptide 1 (alpha subunit, 56kDa); General transcription factor IIE 56 kDa subunit; General transcription factor IIE subunit 1; general transcription factor IIE, polypeptide 1 (alpha subunit); general transcription factor IIE, polypeptide 1 (alpha subunit, 56kDa); general transcription factor IIE, polypeptide 1, alpha 56kDa; TFIIE alpha subunit; TFIIE-alpha; Transcription initiation factor IIE subunit alpha
基因别名: 2610024P03Rik; 56kDa; AI303800; FE; GTF2E1; TF2E1; TFIIE-A
UniProt ID: (Human) P29083, (Mouse) Q9D0D5
Entrez Gene ID: (Human) 2960, (Rat) 303918, (Mouse) 74197
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
viral process
snRNA transcription from RNA polymerase II promoter
biological_process
transcription, DNA-templated
