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Invitrogen
GUCY1B2 Polyclonal Antibody
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GUCY1B2 Antibody (PA5-103135) in ICC/IF
Immunocytochemistry analysis of GUCY1B2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with GUCY1B2 polyclonal antibody (Product # PA5-103135) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-103135
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human GUCY1B2(Accession O75343), corresponding to amino acid residues P581-L617.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total GCS beta 2.
靶标信息
Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase. The soluble form, known as GCS or sGC, act as receptors for nitric oxide. The membrane-bound receptor form, known as GC, are peptide hormone receptors. GCS is a cGMP-synthesizing enzyme, which is the major receptor for the neurotransmitter nitric oxide (NO). It plays a crucial role in smooth muscle contractility, platelet reactivity and neurotransmission. GCS is a heme containing heterodimer, consisting of one 72 kDa alpha subunit and one 65 kDa beta subunit. The heme moeity mediates NO activation, and this heme group also binds carbon monoxide, which weakly stimulates the enzyme.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GCS beta2; GCS-beta-2; GCS-beta2; Guanylate cyclase soluble beta 2 (GTP pyrophosphate - lyase); Guanylate cyclase soluble subunit beta-2; Guanylate cyclase, soluble, beta 2 (GTP pyrophosphate - lyase); soluble guanylate cyclase beta 2a; soluble guanylate cyclase beta 2b; soluble guanylyl cyclase beta2 subunit
基因别名: B930025B03; GC-SB2; Gucy1b2; Gucy1b2a; Gucy1b2b; SGC
UniProt ID: (Rat) P22717
Entrez Gene ID: (Human) 2974, (Rat) 25206, (Mouse) 239134
