Invitrogen

GZF1 Polyclonal Antibody

Name: GZF1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-34375

高级验证

2个已发表图片

1 篇参考文献

查看其他4个GZF1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

GZF1 Antibody in Immunohistochemistry (IHC)

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GZF1 Antibody (PA5-34375) in IHC

Immunohistochemistry of GZF1 in human kidney tissue with GZF1 Polyclonal Antibody (Product # PA5-34375) at 2.5 µg/mL.

Product Image Gallery

GZF1 Antibody — Figure 4. Identification of the sequence differences in the IMiD-dependent binding region of both CRBN and SALL4 in specific species. ( A ) Close-up view of the beta-hairpin loop region of Ck1a (CSNK1A1) interacting with CRBN and lenalidomide (PDB: 5fqd) highlighting the additional bulkiness of the V388I mutation (PDB: 4ci1) present in mouse and rat CRBN. CSNK1A1 and lenalidomide are depicted as stick representations in magenta and yellow, respectively, the Ile391 of mouse CRBN corresponding to human Val388 is depicted as a stick representation in cyan, and CRBN is depicted as a surface representation. ( B ) TR-FRET: titration of DDB1[delta]B-hsCRBN Spy-BodipyFL , or DDB1[delta]B-hsCRBN V388I Spy-BodipyFL to biotinylated hsSALL4 ZnF1-2 at 100 nM, and terbium-streptavidin at 4 nM in the presence of 50 uM pomalidomide or DMSO. ( C ) mES cells were treated with increasing concentrations of thalidomide and pomalidomide or DMSO as a control. Following 24 h of incubation, SALL4 and GAPDH protein levels were assessed by western blot analysis. ( D ) mES cells constitutively expressing Flag-hsCRBN were treated with increasing concentrations of thalidomide. Following 24 h of incubation, ZFP91 and GAPDH protein levels were assessed by western blot analysis. ( E ) As in ( C ), but measuring GZF1 and GAPDH protein levels. ( F ) As in ( C ), but measuring SALL4, hsCRBN (alpha-Flag), and GAPDH protein levels. ( G ) Kelly cells were transiently transfected with Flag-hsSALL4, Flag-mmSALL4, or Flag-mm {TM}

Immunogen image

Immunogen Image GZF1 Polyclonal Antibody

产品信息

PA5-34375

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

2.5 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

An 18 amino acid synthetic peptide near the carboxy terminus of human GZF1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2551727

产品详细信息

A suggested positive control is human heart tissue lysate.

PA5-34375 can be used with blocking peptide PEP-1418.

靶标信息

The GDNF-inducible zinc finger protein 1 (GZF1) is a sequence-specific transcriptional repressor with a BTB/POZ domain and ten zinc finger motifs whose expression is required for renal branching morphogenesis during kidney development. GZF1 binds to the 5'regulatory region of the homeodomain protein HOXA10, suggesting that GZF1 may play a role in morphogenesis other than kidney development. Recent experiments have indicated that GZF1 associates with nucleolin and this association is mediated by the first four zinc finger motifs of GZF1. It is thought that Nucleolin modulates the subcellular localization of GZF1 as well as its transcriptional repressor activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。