Invitrogen

Galectin 9 Monoclonal Antibody (9M1-3), Functional Grade, eBioscience™

Name: Galectin 9 Monoclonal Antibody (9M1-3), Functional Grade, eBioscience\u2122
Brand: Invitrogen
SKU: 16-9116-85

1 篇参考文献

查看其他25个Galectin 9抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

产品信息

16-9116-85

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

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功能检测 (Functional)

Assay-Dependent

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

9M1-3

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2573123

产品详细信息

Description: This 9M1-3 monoclonal antibody reacts with human Galectin-9. Galectin-9 is one of fifteen members of the galectin family of glycan-binding proteins. It is expressed on activated T helper cells and Foxp3+ T regulatory cells and plays a critical role in the regulation of immune function. Interaction of Galectin-9 with its ligand, TIM3, induces apoptosis in Th1 and Th17, inhibits Th17 polarization and IFN gamma expression, and drives Treg expansion. Galectin-9 is also thought to contribute to the survival and expansion of some tumors by inhibiting the anti-tumor response and promoting immune evasion. Along with Galectin-1 and Galectin-3, high levels of Galectin-9 expression are associated with poor cancer prognosis, making these potential therapeutic targets.

Applications Reported: This 9M1-3 antibody has been reported for use in flow cytometric analysis and functional assays. (Fluorochrome-conjugated 9M1-3 is recommended for use in intracellular flow cytometry).

Applications Tested: This 9M1-3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H and RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Ecalectin; Gal-9; Gal9; Galectin-9; Galectin9; HOM HD 21; lectin, galactoside binding soluble 9; lectin, galactoside-binding, soluble, 9; Tumor antigen HOM-HD-21; urate transporter/channel protein

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基因别名: HUAT; LGALS9; LGALS9A

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UniProt ID: (Human) O00182

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Entrez Gene ID: (Human) 3965

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功能

signal transducer activity galactose binding enzyme binding carbohydrate binding disaccharide binding extracellular matrix protein

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