Invitrogen

Galectin 9 Monoclonal Antibody (9M1-3), Functional Grade, eBioscience™

产品信息

16-9116-85

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

查看1篇已发表文章 1篇已发表文章

功能检测 (Functional)

Assay-Dependent

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

9M1-3

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573123

产品详细信息

Description: This 9M1-3 monoclonal antibody reacts with human Galectin-9. Galectin-9 is one of fifteen members of the galectin family of glycan-binding proteins. It is expressed on activated T helper cells and Foxp3+ T regulatory cells and plays a critical role in the regulation of immune function. Interaction of Galectin-9 with its ligand, TIM3, induces apoptosis in Th1 and Th17, inhibits Th17 polarization and IFN gamma expression, and drives Treg expansion. Galectin-9 is also thought to contribute to the survival and expansion of some tumors by inhibiting the anti-tumor response and promoting immune evasion. Along with Galectin-1 and Galectin-3, high levels of Galectin-9 expression are associated with poor cancer prognosis, making these potential therapeutic targets.

Applications Reported: This 9M1-3 antibody has been reported for use in flow cytometric analysis and functional assays. (Fluorochrome-conjugated 9M1-3 is recommended for use in intracellular flow cytometry).

Applications Tested: This 9M1-3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H and RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity.

Cancer cell

Hutton C,Heider F,Blanco-Gomez A,Banyard A,Kononov A,Zhang X,Karim S,Paulus-Hock V,Watt D,Steele N,Kemp S,Hogg EKJ,Kelly J,Jackstadt RF,Lopes F,Menotti M,Chisholm L,Lamarca A,Valle J,Sansom OJ,Springer C,Malliri A,Marais R,Pasca di Magliano M,Zelenay S,Morton JP,Jørgensen C

16-9116-85 was used in Flow Cytometry, Functional Assay to reveal two functionally distinct pancreatic fibroblast lineages and highlight the importance of mesenchymal and immune cell interactions in restricting tumor growth.

Mon Sep 13 00:00:00 EDT 2021

生物信息学

蛋白别名: Ecalectin; Gal-9; Gal9; Galectin-9; Galectin9; HOM HD 21; lectin, galactoside binding soluble 9; lectin, galactoside-binding, soluble, 9; Tumor antigen HOM-HD-21; urate transporter/channel protein

查看更多收起

基因别名: HUAT; LGALS9; LGALS9A

查看更多收起

UniProt ID: (Human) O00182

查看更多收起

Entrez Gene ID: (Human) 3965

查看更多收起

功能

signal transducer activity galactose binding enzyme binding carbohydrate binding disaccharide binding extracellular matrix protein

参与通路