Invitrogen

Gelsolin Polyclonal Antibody

Name: Gelsolin Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-79332

查看其他30个Gelsolin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (8)

Gelsolin Antibody in Immunocytochemistry (ICC/IF)

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Gelsolin Antibody (PA5-79332) in ICC/IF

Immunocytochemistry analysis of Gelsolin using anti-Gelsolin antibody (Product # PA5-79332). Gelsolin was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Gelsolin antibody (Product # PA5-79332) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained w... View More

Gelsolin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Gelsolin Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image Gelsolin Polyclonal Antibody

产品信息

PA5-79332

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

0.5-1 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of human Gelsolin (763-775aa WDDDYWSVDPLDR).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide, 0.05mg thimerosal

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2746448

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human HepG2 whole cell, human THP-1 whole cell, human U251 whole cell, monkey COS-7 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell. IHC: Human Lung Cancer tissue, Human Intestinal Cancer tissue, Rat Cardiac Muscle tissue. ICC/IF: U20S cell, NIH3T3 Cell. Flow: U87 cell.

靶标信息

The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。