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Bethyl Laboratories
Glu-Glu Tag Polyclonal Antibody
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Glu-Glu Tag Antibody (A190-110A) in WB
Detection of Glu-Glu-tagged protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-Glu-Glu antibody (Product # A190-110A) used for WB at 0.2 µg/mL (1:5,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
产品信息
A190-110A
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide representing amino acid residues 314 - 319 (EYMPME) of middle T antigen of mouse polyomavirus conjugated to KLH.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 6.8 to 7.4
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Goats were immunized with a synthetic peptide representing amino acid residues 314 - 319 (EYMPME) of middle T antigen of mouse polyomavirus conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
靶标信息
The Glu-Glu tag, also known as the EYMPME sequence, is a short peptide epitope tag consisting of two glutamic acid (Glu) residues interspersed with various amino acids. It is typically incorporated into the N-terminus of proteins through subcloning into vectors such as the pFASTBAC vector. This tag is particularly useful in recombinant protein expression and purification processes. It facilitates the detection and isolation of tagged proteins via specific antibodies that recognize the Glu-Glu sequence. The Glu-Glu tag can be utilized in various systems, including baculovirus expression in insect cells, and provides efficient means for studying protein-protein interactions, localization, and function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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