Invitrogen

GluR2 Monoclonal Antibody (6C4)

Name: GluR2 Monoclonal Antibody (6C4)
Brand: Invitrogen
SKU: 32-0300
Availability: InStock

高级验证

15个已发表图片

35 篇参考文献

查看其他33个GluR2抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

GluR2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 20

GluR2 Antibody (32-0300) in ICC/IF

Immunofluorescence analysis of GluR2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GluR2 (6C4) Mouse Monoclonal Antibody (Product # 32-0300) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28... View More

GluR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GluR2 Antibody in Immunohistochemistry (IHC)

GluR2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

GluR2 Antibody — Figure 7 Lithium treatment significantly increases surface GluR1/GluR2 in the hippocampus, but not frontal cortex in vivo Mice were treated with lithium or control chow for 1.5 days. Following treatment, mice were decapitated and brains were rapidly removed. Hippocampal (HP) and frontal cortex (FC) tissues were isolated and the BS3 cross-linking procedure was performed to identify surface GluR1/GluR2 tetramers. Western blot analyses were performed with anti-GluR1 and anti-GluR2 antibodies. Representative images for cross-linked and intracellular GluR1 and GluR2 by western blot analysis (a, b). Densitometry analysis: Lithium significantly enhanced surface cross-linked GluR1 and GluR2 in hippocampus, but not in prefrontal cortex vivo. N=6 mice per group (b-e). * p <0.05 {TM}

Immunogen image

Immunogen Image GluR2 Monoclonal Antibody (6C4)

产品信息

32-0300

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

查看17篇已发表文章 17篇已发表文章

免疫组化 (IHC)

查看7篇已发表文章 7篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20-1:100

免疫细胞化学 (ICC/IF)

2 µg/mL

查看7篇已发表文章 7篇已发表文章

酶联免疫吸附实验 (ELISA)

0.5-5 µg/mL

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

功能检测 (Functional)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human, Mouse, Non-human primate, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

6C4

抗原

Fusion protein containing amino acids 175-430 from the N-terminal region of GluR2.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

100 µg

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533058

靶标信息

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5 -methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct Glutamate Receptor Subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR2 subunit is thought to play a key role in forms of synaptic plasticity such as LTD.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: AMPA selective glutamate receptor; AMPA-selective glutamate receptor 2; GluR-2; GluR-B; GluR-K2; glutamate receptor; Glutamate receptor 2; glutamate receptor B; Glutamate receptor ionotropic, AMPA 2; OTTHUMP00000165324; OTTHUMP00000220942; OTTHUMP00000220943

查看更多收起

基因别名: GluA2; Glur-2; GluR-B; GluR-K2; GLUR2; GRIA2

查看更多收起

UniProt ID: (Rat) P19491

查看更多收起

Entrez Gene ID: (Mouse) 14800, (Rat) 29627

查看更多收起

功能