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Invitrogen
F(ab)-Goat anti-Human IgG Fc Superclonal™ Secondary Antibody
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Human IgG Fc Secondary Antibody (SA5-10372) in ICC/IF
Immunofluorescence analysis of F(ab) Goat anti-Human IgG Fc Recombinant Secondary Antibody (Product # SA5-10372) was performed on fixed and permeabilized T-47D cells. Cells were blocked with 2% BSA for 1 hour at room temperature and probed with the primary antibody MUC-1/Gatipotuzumab Recombinant Human Monoclonal Antibody (Product # MA5-42157, 1:500) in 0.1% BSA overnight at 4 degrees Celsius. This was dete... View More
产品信息
SA5-10372
应用
建议稀释比
免疫印迹 (WB)
1:1,000
免疫细胞化学 (ICC/IF)
1:1,000
酶联免疫吸附实验 (ELISA)
1:1,000
产品规格
种属反应
Human
宿主/亚型
Goat
/ IgG, F(ab)
Expression System
Expi293
分类
Recombinant Superclonal
类型
Secondary Antibody
抗原
Purified Human IgG
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
靶标
IgG Fc
抗体形式
F(ab) Fragment
产品详细信息
F(ab)-Goat anti-Human IgG Fc, Superclonal™ Recombinant Secondary Antibody specifically detects all subtypes of Human IgG and is very specific to Fc region of Human IgG. It has moderate cross reactivity to Rat IgG and Monkey IgG. It showed no cross-reactivity to immunoglobulins tested from other species.
Smaller F(ab) antibody fragments offer several benefits over whole IgGs, due to their smaller size (~50 kD vs. ~150 kD) and the absence of the Fc region. The smaller size is expected to allow conjugated F(ab) fragments to better penetration into tissue sections and improved staining in applications such as IHC. The lack of Fc region results in reduced nonspecific background staining when staining cells/ tissues with Fc receptors (such as immune cells) and other Fc interaction-related interference.
Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
