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Invitrogen
Goat anti-Mouse IgG1 Superclonal™ Secondary Antibody
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Mouse IgG1 Secondary Antibody (SA5-10373) in ICC/IF
Immunofluorescence analysis of Goat anti-Mouse IgG1, Superclonal™ Recombinant Secondary Antibody (Product # SA5-10373) was performed on fixed and permeabilized HeLa cells blocked with 2% BSA at room temperature and stained with beta Tubulin Monoclonal Antibody (Product # 32-2600, 1:500) antibody in 0.1% BSA overnight at 4-degree Celsius. Detection was performed using Goat anti-Mouse IgG1, Superclonal™ Recom... View More
产品信息
SA5-10373
应用
建议稀释比
免疫印迹 (WB)
1:2,000-1:4,000
免疫组化（石蜡） (IHC (P))
1:500
免疫细胞化学 (ICC/IF)
1:1,000
酶联免疫吸附实验 (ELISA)
1:1,000-1:4,000
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Secondary Antibody
抗原
Purified Mouse IgG
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
靶标
IgG1
抗体形式
Whole Antibody
产品详细信息
Goat anti-Mouse IgG1 Recombinant Superclonal™ Secondary Antibody specifically detects the Fc region of mouse IgG1 with no cross-reactivity to mouse IgG2a, mouse IgG2b or immunoglobulins from other species.
The sensitivity and specificity of each lot is confirmed using ELISA.
Superclonal™ secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency.
It is recommended that the antibody is carefully titrated for optimal performance in the assay of interest.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
