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Invitrogen
Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 350
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Mouse IgG2a Cross-Adsorbed Secondary Antibody (A-21130) in ICC/IF
Immunofluorescence analysis of Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 350 (Product # A-21130) was performed using SH-SY5Y (positive model) and HeLa (negative model) cells stained with beta-3 Tubulin Monoclonal Antibody (2G10) (Product # MA1-118). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% ... View More
产品信息
A-21130
应用
建议稀释比
免疫组化 (IHC)
1-10 µg/mL
免疫细胞化学 (ICC/IF)
1-10 µg/mL
流式细胞分析 (Flow)
1-10 µg/mL
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Mouse IgG2a
偶联物
激发/发射光谱
343/441 nm
查看光谱
形式
Liquid
浓度
2 mg/mL
纯化类型
purified
保存液
PBS, pH 7.5
内含物
5mM sodium azide
保存条件
4°C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标
IgG2a
交叉吸附
Against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins and mouse isotypes IgG1, IgG2b and IgG3 prior to conjugation
抗体形式
Whole Antibody
产品详细信息
Product will be shipped at Room Temperature.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
