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Invitrogen
F(ab)-Goat anti-Mouse IgG2b Recombinant Secondary Antibody, Alexa Fluor™ Plus 488
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Mouse IgG2b Secondary Antibody (SA5-10382-AFP488) in ICC/IF
Immunofluorescence analysis of F(ab)-Goat anti-Mouse IgG2b Recombinant Secondary Antibody, Alexa Fluor™ Plus 488 (Product # SA5-10382-AFP488) was performed on fixed and permeabilized HeLa cells blocked with SuperBlock™ Blocking Buffer (Product # 37515) at room temperature and stained with TUBB4 Monoclonal Antibody (OTI5C1) (Product # TA503129, 1:500) in SuperBlock™ Blocking Buffer (Product # 37515, 1:20) ov... View More
产品信息
SA5-10382-AFP488
应用
建议稀释比
免疫组化（石蜡） (IHC (P))
1:500
免疫细胞化学 (ICC/IF)
1:1,000
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG, F(ab)
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Secondary Antibody
克隆号
27H27L7
抗原
Purified Mouse IgG
偶联物
激发/发射光谱
493/518 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
proprietary buffer, pH 6.3-7.3
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
靶标
IgG2b
抗体形式
F(ab) Fragment
产品详细信息
F(ab)-Goat anti-Mouse IgG2b Recombinant Secondary Antibody, Alexa Fluor™ Plus 488 is designed to specifically detect the mouse IgG2b isotype showing no detectable reactivity with immunoglobulins from any other tested species.
This Fab fragment antibody is expressed in vitro and purified as a recombinant construct. It is also engineered to deliver enhanced stability and sensitivity. The advantages of recombinant antibodies include-better specificity, animal origin-free formulation, and lot-to-lot consistency.
F(ab) fragments offer several advantages over full-length IgGs due to their smaller molecular size (~50 kDa vs. ~150 kDa), such as improved tissue penetration. The absence of the Fc region reduces nonspecific binding and background staining in Fc receptor-expressing cells and tissues, while also enabling increased flexibility in multiplexing assays.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
