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Invitrogen
Goat anti-Rabbit IgG Fc Superclonal™ Secondary Antibody, Alexa Fluor™ 488
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Rabbit IgG Fc Secondary Antibody (SA5-10378-AF488) in ICC/IF
Immunofluorescence analysis of Goat anti-Rabbit IgG Fc Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ 488 (Product # SA5-10378-AF488) was performed on fixed and permeabilized HeLa cells blocked with SuperBlock™ Blocking Buffer (Product # 37515) at room temperature and stained with H3K4me3 Recombinant Rabbit Monoclonal Antibody (14H24L5) (Product # 703849, 1:250) in SuperBlock™ Blocking Buffer (Pr... View More
产品信息
SA5-10378-AF488
应用
建议稀释比
免疫组化（石蜡） (IHC (P))
1:250
免疫细胞化学 (ICC/IF)
1:1,000
产品规格
种属反应
Rabbit
宿主/亚型
Goat
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Secondary Antibody
抗原
Purified Rabbit IgG
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
proprietary buffer, pH 6.3-7.3
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
靶标
IgG Fc
抗体形式
Whole Antibody
产品详细信息
The Goat anti-Rabbit IgG Fc Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ 488 is specific to Fc region of Rabbit IgG with moderate cross reactivity to Monkey IgG. It showed no cross-reactivity to any other immunoglobulins tested from other species.
The sensitivity and specificity of each lot is confirmed using ELISA.
Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency.
Protein conjugates made with Alexa Fluor™ dyes produce fluorescence output that surpasses that of other spectrally similar fluorophore-labeled proteins. Alexa Fluor™ dyes are available in a wide selection of fluorescent colors and are more photostable than most other dyes. Photostability is a key characteristic of Alexa Fluor dye™ conjugates, allowing more time for image capture. In addition to their exceptional brightness and the wide selection of dyes across the spectrum, you'll get photostability you can rely on with Alexa Fluor™ dyes and dye conjugates.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
