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Rabbit IgG (Heavy chain) Secondary Antibody (A27034) in ICC/IF
Immunofluorescence analysis of Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ 488 (Product # A27034) was performed using MCF 10A (positive model) and A-431 (negative model) in cells stained with Vimentin Polyclonal antibody (Product # PA5-27231). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, ... View More
产品信息
A27034
应用
建议稀释比
免疫印迹 (WB)
1:2,000-1:5,000
免疫组化（冰冻） (IHC (F))
1:2,000
免疫细胞化学 (ICC/IF)
1:250-1:2,000
流式细胞分析 (Flow)
1-5 µg/mL
产品规格
种属反应
Rabbit
宿主/亚型
Goat
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Secondary Antibody
抗原
Recombinant full-length protein
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Gravity column chromatography
保存液
PBS
内含物
5mM sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
靶标
IgG (Heavy chain)
抗体形式
Whole Antibody
产品详细信息
The sensitivity and specificity of each lot is confirmed using ELISA.
Minimal cross-reactivity with mouse, rat, human, bovine, guinea pig and donkey IgG is observed.
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency.
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
