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Invitrogen
F(ab')2-Goat anti-Rat IgG (H+L) Secondary Antibody, FITC, eBioscience™
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Rat IgG (H+L) Secondary Antibody (11-4017-82) in Flow
Staining of BALB/c splenocytes with Rat IgG2a K Isotype Control Purified (Product # 14-4321-82) (blue histogram) or Anti-Mouse CD4 Purified (clone RM4-5, a rat IgG2a isotype, Product # 14-0042-82) (left) or Anti-Mouse CD8a Purified (clone 53-6-7, a rat IgG2a isotype, Product # 14-0081-82) (right) followed by 0.125 µg of F (ab')2 Anti-Rat IgG FITC (purple histogram). Cells in the lymphocyte gate were used fo... View More
产品信息
11-4017-82
应用
建议稀释比
流式细胞分析 (Flow)
0.25 µg/test
产品规格
种属反应
Rat
宿主/亚型
Goat
/ Ig
分类
Polyclonal
类型
Secondary Antibody
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标
IgG
抗体形式
F(ab')2 Fragment
产品详细信息
Description: The goat anti-rat IgG polyclonal antibody reacts with the heavy and light chains of rat IgG, with minimal cross reactivity to human, mouse, bovine, goat, hamster, and rabbit serum proteins. Reactivity has been shown to all IgG subclasses: IgG1, IgG2a, IgG2b, IgG2c, and some crossreactivity to rat IgM. The antibody is a F(ab')2 fragment which contains the 2 Fab domains still linked through the hinge region of the immunoglobulin. Removal of the Fc region helps to reduce issues typically found with Fc-mediated binding.
This polyclonal has been validated as a secondary reagent (its ability to recognize cells stained with a rat IgG followed by this polyclonal antibody).
Applications Reported: This poly antibody has been reported for use in flow cytometric analysis.
Applications Tested: This poly antibody has been tested by flow cytometric analysis of mouse splenocytes stained with a primary antibody of the rat IgG isotype. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
