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Invitrogen
Goat anti-Rat IgG2b Recombinant Secondary Antibody, Alexa Fluor™ Plus 680
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Rat IgG2b Secondary Antibody (SA5-10377-AFP680) in ICC/IF
Immunofluorescence analysis of Goat anti-Rat IgG2b, Recombinant Secondary Antibody, Alexa Fluor™ Plus 680 (Product # SA5-10377-AFP680) was performed on fixed and permeabilized HeLa cells. Cells were blocked with SuperBlock™ Blocking Buffer for 1 hour at room temperature and probed with hnRNP UL1 Monoclonal Antibody (EAP 4A11) (Product # 14-9806-82, 1:400) overnight at 4 degrees Celsius. This was detected by... View More
产品信息
SA5-10377-AFP680
应用
建议稀释比
免疫组化（石蜡） (IHC (P))
1:500
免疫细胞化学 (ICC/IF)
1:1,000
产品规格
种属反应
Rat
宿主/亚型
Goat
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Secondary Antibody
克隆号
10H38L66
抗原
Purified Rat IgG
偶联物
激发/发射光谱
687/704 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
proprietary buffer, pH 6.3-7.3
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
靶标
IgG2b
抗体形式
Whole Antibody
产品详细信息
Goat anti-Rat IgG2b, Recombinant Secondary Antibody specifically detects the Fc portion of rat IgG2b isotype and shows strong reactivity to total rat IgG. It has minimal to no cross-reactivity towards other rat IgG isotypes and immunoglobulins tested from other species.
Alexa Fluor™ Plus conjugated recombinant antibodies provide bright signals and high signal-to-noise for your critical experiments.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
