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Invitrogen
Goat anti-Rat IgM Secondary Antibody, Biotin
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产品信息
31832
应用
建议稀释比
免疫印迹 (WB)
1:20,000-1:400,000
免疫组化 (IHC)
1:500-1:5,000
免疫细胞化学 (ICC/IF)
1:500-1:5,000
流式细胞分析 (Flow)
1:200-1:1,000
酶联免疫吸附实验 (ELISA)
1:20,000-1:400,000
免疫沉淀 (IP)
1:500-1:5,000
产品规格
种属反应
Rat
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
形式
Lyophilized
浓度
1.6 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.6, with 15mg/mL BSA
内含物
0.05% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标
IgM
抗体形式
Whole Antibody
产品详细信息
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31832 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31832 reacts with the heavy chain of rat IgM, but not with IgG or with non-immunoglobulin serum proteins. However, antibodies may cross-react with IgM from other species.
Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Reconstitute with 2.0 mL of distilled water.
Country of Origin: USA
靶标信息
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
