Invitrogen

Grancalcin Polyclonal Antibody

Name: Grancalcin Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-95598
Availability: InStock

查看其他9个Grancalcin抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (6)

Grancalcin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

Grancalcin Antibody (PA5-95598) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of Grancalcin in Hela cells using Grancalcin Polyclonal Antibody (Product # PA5-95598). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. Cy3 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution... View More

Grancalcin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Grancalcin Antibody in Western Blot (WB)

Grancalcin Antibody in Flow Cytometry (Flow)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

Immunogen image

Immunogen Image Grancalcin Polyclonal Antibody

产品信息

PA5-95598

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Grancalcin/GCA recombinant protein (Position: A42-I217).

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2807400

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human MCF-7 whole cell, human HL-60 whole cell, human HEL whole cell, rat testis tissue, mouse testis tissue. IHC: human bladder urothelial carcinoma tissue, human colorectal adenocarcinoma tissue, human lung cancer tissue. ICC/IF: Hela cell. Flow: HL-60 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。