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Invitrogen
Green Renilla luciferase Polyclonal Antibody
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Green Renilla luciferase Antibody (PA1-180) in ICC/IF
Immunofluorescent analysis of Green Renilla luciferase (from the Sea pansy Renilla reniformis) in untransfected (UT, left panel) and pCMV-Green Renilla (GR) luciferase transfected (green, right panel) 293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 2% Blocker BSA (Product # 37525) for 15 minutes at room temperature.... View More
产品信息
PA1-180
应用
建议稀释比
已发表文章
产品规格
种属反应
Renilla
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Purified full length recombinant Green Renilla luciferase expressed in E. coli.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS with 30% glycerol, 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Western blot analysis of PA1-180 detects a prominent ~36 kDa protein in Green Renilla luciferase expressing cells. Additional unspecific bands are also detected in control cells with increased exposure time. This antibody has also been successfully tested in IP, Immunofluorescence, and neutralization assays.
Green Renilla luciferase activity can be measured using the Thermo Fisher Firefly Luciferase Flash or Glow Assay Kits or Luciferase Dual Assay Kits.
靶标信息
The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly. The Renilla Flash and Glow Assay Kit reagents allow for Glow (stabilized signal with ~3 hours halflife) or Flash type bioluminescence experiments and are optimized for best results with Thermo Scientific Green Renilla Luc Plasmids. Bioluminescence provides researchers with a non-radioactive assay for measuring transcriptional activity of regulatory elements. Reporter activity can be used to determine promoter activity, to study upstream pathway activity in the cell, or to determine effect of activators or inhibitors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
