Invitrogen

Grim-19 Monoclonal Antibody (1A8), eBioscience™

Name: Grim-19 Monoclonal Antibody (1A8), eBioscience\u2122
Brand: Invitrogen
SKU: 14-9937-82

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8 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
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Grim-19 Antibody (14-9937-82) in WB

Immunoblotting of reduced HeLa cell lysate with 5 µg/mL of Anti-Grim-19 Purified antibody. Bands were visualized using Anti-Mouse IgG HRP.

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产品信息

14-9937-82

应用

建议稀释比

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免疫印迹 (WB)

5 µg/mL

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免疫组化（石蜡） (IHC (P))

Assay-Dependent

免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

Assay-Dependent

流式细胞分析 (Flow)

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其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

1A8

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10597591

产品详细信息

Description: This 1A8 monoclonal antibody reacts with human, mouse, and rat Grim-19. This 16 kDa protein is expressed in many human tissues, especially in the heart and skeletal muscle. Lower expression levels of Grim-19 can be observed in the liver, kidney, and brain. Originally described as a nuclear protein, reports indicate that Grim-19 is primarily localized to mitochondria as an essential component of complex I of the electron transport chain. Grim-19 expression can be induced by interferon-beta and all-trans-retinoic acid in tumor cell lines, leading to cell death. Finally, Grim-19 has been shown to bind the transactivation domain of STAT3 and inhibit its transcriptional activity. The molecular mechanisms of how Grim-19 regulates STAT3 within the cell remains under active investigation.

Applications Reported: This 1A8 antibody has been reported for use in immunoblotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This 1A8 antibody has been tested by immunoblotting of HeLa cell lysate. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

A novel gene associated with Retinoid-IFN-induced Mortality (GRIM) GRIM-19 gene was identified. Antisense expression of GRIM-19 confers a strong resistance against IFN/RA-induced death by reducing the intracellular levels of GRIM-19 protein. Overexpression of GRIM-19 enhances cell death in response to IFN/RA. GRIM-19 is primarily a nuclear protein whose expression is induced by the IFN/RA combination. These data indicate that GRIM-19 is a novel cell death-regulatory molecule.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Cell d; Cell death regulatory protein GRIM-19; cell death-regulatory protein GRIM19; CI-B16.6; complex I B16.6 subunit; Complex I-B16.6; FLJ58045; FLJ59191; gene associated with retinoic and IFN-induced mortality 19 protein; Gene associated with retinoic and interferon-induced mortality 19 protein; gene associated with retinoic-interferon-induced mortality 19 protein; genes associated with retinoid-IFN-induced mortality 19; GRIM-19; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13; NADH-ubiquinone oxidoreductase B16.6 subunit

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基因别名: 2700054G14Rik; AU022060; B16.6; CDA016; CGI-39; GRIM-19; GRIM19; NDUFA13

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UniProt ID: (Human) Q9P0J0, (Mouse) Q9ERS2

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Entrez Gene ID: (Rat) 100911483, (Human) 51079, (Mouse) 67184

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功能

NADH dehydrogenase activity protein binding ATP binding NADH dehydrogenase (ubiquinone) activity dehydrogenase oxidoreductase metabolite interconversion enzyme

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