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Invitrogen
HA Tag Monoclonal Antibody (5B1D10)
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HA Tag Antibody (32-6700) in ICC/IF
Immunofluorescent analysis of HA Tag was performed using HA-H3 construct transfected in HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HA Tag Monoclonal Antibody (5B1D10) (Product # 32-6700) at 2 µg/mL and Histone H3 Rabbit Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) ... View More
产品信息
32-6700
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
已发表种属
C. elegans,
Tag
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
5B1D10
抗原
A synthetic peptide corresponding to the nine amino acid sequence HA-tag (YPYDVPDYA).
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This monoclonal antibody reacts specifically with HA-tagged fusion proteins and reactivity with various HA-tagged fusion proteins has been confirmed by western blotting; positive control used were HA-tagged JNK-1 transfected cell lysates.
靶标信息
HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
