Invitrogen

HDAC9 Recombinant Rabbit Monoclonal Antibody (JU30-44)

Name: HDAC9 Recombinant Rabbit Monoclonal Antibody (JU30-44)
Brand: Invitrogen
SKU: MA5-32820

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (7)
已发表图片 (1)

HDAC9 Antibody in Immunocytochemistry (ICC/IF)

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HDAC9 Antibody (MA5-32820) in ICC/IF

Immunocytochemical analysis of HDAC9 in SH-SY5Y cells using a HDAC9 Monoclonal antibody (Product # MA5-32820) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

HDAC9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-32820

应用

建议稀释比

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免疫印迹 (WB)

1:2,000

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免疫组化（石蜡） (IHC (P))

1:500-1:2000

免疫细胞化学 (ICC/IF)

1:50-1:200

免疫沉淀 (IP)

1:10-1:50

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JU30-44

抗原

Synthetic peptide within Human HDAC9 aa 1-50

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2810096

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is a critical component of transcriptional regulation and the acetylation of nucleosomal histones is a major source of this remodeling. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Several mammalian proteins function as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Conversely, the deacetylation of histones is associated with transcriptional silencing. The histone deacetylases (HDAC) include HDAC1-9. HDAC9 and HDAC9a are two alternatively spliced isoforms of HDAC9. HDAC9a is 132 amino acids shorter than HDAC9, but both isoforms contain the HDAC catalytic domain, remain capable of deacetylase activity and repress myoctye enhancer-binding factor 2-mediated transcription. HDAC9 and HDAC9a are expressed in brain, skeletal muscle, kidney, placenta and pancreas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: DKFZp779K1053; HD7; HD9; hdac-9; histone deacetylase 4/5-related protein; Histone deacetylase 7B; Histone deacetylase 9; Histone deacetylase-related protein; MEF-2 interacting transcription repressor (MITR) protein; MEF2-interacting transcription repressor MITR

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基因别名: HD7; HD7b; HD9; HDAC; HDAC7; HDAC7B; HDAC9; HDAC9B; HDAC9FL; HDRP; KIAA0744; MITR

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UniProt ID: (Human) Q9UKV0

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Entrez Gene ID: (Human) 9734

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功能