Invitrogen

HES5 Recombinant Rabbit Monoclonal Antibody (4S4U7)

查看其他11个HES5抗体

问题与答案

问题与答案

抗体检测数据 (3)

HES5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HES5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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HES5 Antibody (MA5-38214) in IHC (P)

Immunohistochemistry analysis of HES5 in paraffin-embedded human lung cancer. Samples were incubated with HES5 Monoclonal antibody (Product # MA5-38214) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

HES5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HES5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HES5 Antibody in Western Blot (WB)

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HES5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of HES5 in paraffin-embedded human lung cancer. Samples were incubated with HES5 Monoclonal antibody (Product # MA5-38214) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

HES5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of HES5 in paraffin-embedded rat ovary. Samples were incubated with HES5 Monoclonal antibody (Product # MA5-38214) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

HES5 Antibody in Western Blot (WB) — Western blot analysis of HES5 in extracts of various cell lines. Samples were incubated with HES5 Monoclonal antibody (Product # MA5-38214) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunogen image

HES5 Recombinant Rabbit Monoclonal Antibody (4S4U7)

Immunogen Image HES5 Recombinant Rabbit Monoclonal Antibody (4S4U7)

产品信息

MA5-38214

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:6,000

-

免疫组化（石蜡） (IHC (P))

1:100-1:500

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

4S4U7

抗原

Recombinant protein (or fragment)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.9 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Positive Samples: Mouse brain, Mouse spleen, Rat brain, Rat spleen

Immunogen sequence: SPKEKNRLRK PVVEKMRRDR INSSIEQLKL LLEQEFARHQ PNSKLEKADI LEMAVSYLKH SKAFVAAAGP KSLHQDYSEG YSWCLQEAVQ FLTLHAASDT QMKLLYHFQR PPAAPAAPAK EPKAPGAAPP P

靶标信息

Hairy and Enhancer-of-split homologues 5 (HES5) is a helix-loop-helix transcriptional factor that negatively regulates cell differentiation during embryogenesis. It contains one basic helix-loop-helix (bHLH) domain and one Orange domain. HES5 is reported to be a down stream effector of the Notch Signaling Pathway and is expressed in advanced ovarian serous adenocarcinomas as well as in cervical squamous carcinoma. Reports also suggest that HES5 is abundantly expressed in osteoarthritis along with Notch1 and Jagged1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: bHLHb38; Class B basic helix-loop-helix protein 38; Hairy and enhancer of split 5; RP3-395M20.5; Transcription factor HES-5

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基因别名: BHLHB38; Hes-5; HES5

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UniProt ID: (Human) Q5TA89, (Rat) Q03062, (Mouse) P70120

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Entrez Gene ID: (Human) 388585, (Rat) 79225, (Mouse) 15208

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transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding double-stranded DNA binding transcription factor binding protein dimerization activity DNA binding protein binding basic helix-loop-helix transcription factor

negative regulation of transcription from RNA polymerase II promoter chondrocyte differentiation transcription, DNA-templated protein complex assembly cell adhesion Notch signaling pathway smoothened signaling pathway brain development positive regulation of cell proliferation oligodendrocyte development telencephalon development glial cell fate commitment forebrain radial glial cell differentiation neural tube development central nervous system myelination positive regulation of BMP signaling pathway regulation of myelination auditory receptor cell differentiation positive regulation of tyrosine phosphorylation of Stat3 protein auditory receptor cell fate determination camera-type eye development regulation of cell differentiation regulation of auditory receptor cell differentiation negative regulation of neuron differentiation positive regulation of Notch signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade cell maturation positive regulation of smooth muscle cell proliferation astrocyte differentiation negative regulation of astrocyte differentiation negative regulation of oligodendrocyte differentiation regulation of epithelial cell proliferation regulation of neurogenesis cartilage development inner ear receptor stereocilium organization comma-shaped body morphogenesis S-shaped body morphogenesis specification of loop of Henle identity metanephric nephron tubule morphogenesis establishment of epithelial cell polarity neuronal stem cell population maintenance negative regulation of stem cell differentiation negative regulation of pro-B cell differentiation negative regulation of forebrain neuron differentiation negative regulation of inner ear receptor cell differentiation transcription from RNA polymerase II promoter neuron differentiation negative regulation of transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development nervous system development cell differentiation