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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • HIF1A Antibodies

          Invitrogen

          HIF1A Monoclonal Antibody (ESEE122), DyLight™ 488

          1 Published Figure
          2 References
          View all (66) HIF1A antibodies

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          Cite HIF1A Monoclonal Antibody (ESEE122), DyLight™ 488

          • Published Figures (1)
          HIF1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          HIF1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          HIF1A Antibody (MA5-16018) in IHC (P)

          MethodsX 2023 - Fig. 1 IHC staining of U251 tumoroid cytoskeleton with actin (a), autophagosome with LC3 (b, c), and hypoxia with Hif1-alpha following the presented protocol (d-1) compared with the tumoroids underwent conventional intermediate steps of washing and centrifugation (d-2). Tumoroids were counterstained with DAPI. Scale bars are 100 um. Fig. 1 {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          HIF1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HIF1A Monoclonal Antibody (ESEE122), DyLight™ 488

          Product Details

          MA5-16018

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-Dependent
          -

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:5000
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          1:100-1:5000
          -

          Immunocytochemistry (ICC/IF)

          10 µg/mL
          -

          Flow Cytometry (Flow)

          Assay-Dependent
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Bovine, Dog, Human, Mouse, Rat

          Published species

          C. elegans

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          ESEE122

          Immunogen

          Human HIF-1 alpha corresponding to amino acids 329-530.
          View immunogen

          Conjugate

          DyLight™ 488 DyLight™ 488 DyLight™ 488
          • Unconjugated
          • Biotin 
          • HRP
          • Request custom conjugation

          Excitation/Emission Max

          492/519 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.71 mg/mL

          Purification

          Protein G

          Storage buffer

          50mM sodium borate

          Contains

          0.05% sodium azide

          Storage conditions

          4°C, store in dark

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_11157208

          Product Specific Information

          Suggested positive control: Cos7 CoCl2-treated nuclear extract.

          Target Information

          HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: ARNT interacting protein; ARNT-interacting protein; ARNT2; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; hif 1; hif 1a; HIF-1 alpha; HIF-1-alpha; HIF1 alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; Member of PAS protein 1; member of PAS superfamily 1; PAS domain-containing protein 8

          View more View less

          Gene Aliases: AA959795; BHLHE78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; HIF1A; HIF1alpha; MOP1; PASD8

          View more View less

          UniProt ID: (Human) Q16665, (Mouse) Q61221, (Rat) O35800

          View more View less

          Entrez Gene ID: (Bovine) 281814, (Human) 3091, (Dog) 480348, (Mouse) 15251, (Rat) 29560

          View more View less

          Function(s)
          transcription factor activity, sequence-specific DNA binding histone acetyltransferase binding sequence-specific DNA binding protein heterodimerization activity transcription factor activity, transcription factor binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding enzyme binding protein kinase binding ubiquitin protein ligase binding protein complex binding nuclear hormone receptor binding histone deacetylase binding Hsp90 protein binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding signal transducer activity protein dimerization activity double-stranded DNA binding basic helix-loop-helix transcription factor
          Process(es)
          response to hypoxia positive regulation of vascular endothelial growth factor production axonal transport of mitochondrion positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of transcription from RNA polymerase II promoter in response to hypoxia cellular response to hypoxia negative regulation of transcription from RNA polymerase II promoter angiogenesis neural crest cell migration epithelial to mesenchymal transition embryonic placenta development B-1 B cell homeostasis positive regulation of endothelial cell proliferation heart looping positive regulation of neuroblast proliferation connective tissue replacement involved in inflammatory response wound healing outflow tract morphogenesis cardiac ventricle morphogenesis lactate metabolic process regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cellular iron ion homeostasis acute-phase response signal transduction lactation visual learning response to salt stress response to X-ray regulation of gene expression vascular endothelial growth factor production positive regulation of gene expression positive regulation of epithelial cell migration positive regulation of receptor biosynthetic process response to auditory stimulus response to purine-containing compound response to muscle activity positive regulation of macroautophagy neural fold elevation formation cerebral cortex development negative regulation of bone mineralization positive regulation of vascular endothelial growth factor receptor signaling pathway negative regulation of TOR signaling response to estradiol oxygen homeostasis positive regulation of chemokine production cellular response to insulin stimulus regulation of transforming growth factor beta2 production collagen metabolic process embryonic hemopoiesis cellular response to drug positive regulation of insulin secretion involved in cellular response to glucose stimulus hemoglobin biosynthetic process mRNA transcription from RNA polymerase II promoter positive regulation of apoptotic process response to alkaloid regulation of transcription from RNA polymerase II promoter in response to oxidative stress response to estrogen positive regulation of erythrocyte differentiation positive regulation of angiogenesis positive regulation of cell size positive regulation of glycolytic process negative regulation of vasoconstriction negative regulation of growth muscle cell cellular homeostasis positive regulation of hormone biosynthetic process digestive tract morphogenesis positive regulation of smooth muscle cell proliferation positive regulation of nitric-oxide synthase activity cartilage development response to glucocorticoid elastin metabolic process maternal process involved in female pregnancy intestinal epithelial cell maturation response to fungicide epithelial cell differentiation involved in mammary gland alveolus development iris morphogenesis retina vasculature development in camera-type eye regulation of transcription from RNA polymerase II promoter in response to hypoxia positive regulation of chemokine-mediated signaling pathway negative regulation of thymocyte apoptotic process cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to carbon monoxide cellular response to nitrite cellular response to electrical stimulus cellular response to mechanical stimulus cellular response to cobalt ion cellular response to glucose stimulus cellular response to interleukin-1 cellular response to organic cyclic compound cellular response to light stimulus dopaminergic neuron differentiation response to anesthetic cellular response to toxic substance hypoxia-inducible factor-1alpha signaling pathway regulation of cellular response to hypoxia positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway positive regulation of mitophagy regulation of aerobic respiration cellular response to cyanide negative regulation of reactive oxygen species metabolic process negative regulation of mesenchymal cell apoptotic process blood vessel development vasculature development regulation of glycolytic process transcription, DNA-templated regulation of transcription from RNA polymerase II promoter positive regulation of cell proliferation positive regulation of autophagy cell differentiation negative regulation of transcription elongation from RNA polymerase II promoter regulation of cell proliferation glucose homeostasis negative regulation of apoptotic process negative regulation of neuron apoptotic process blood vessel morphogenesis regulation of catalytic activity regulation of thymocyte apoptotic process response to mechanical stimulus response to glucose response to activity response to cobalt ion response to drug cellular response to lipid
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