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Invitrogen
HIPK2 Polyclonal Antibody
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HIPK2 Antibody (PA5-102964) in ICC/IF
Immunocytochemistry analysis of HIPK2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with HIPK2 polyclonal antibody (Product # PA5-102964) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-102964
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human HIPK2(Accession Q9H2X6), corresponding to amino acid residues F15-T65.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total HIPK2.
靶标信息
The HIPK2 gene encodes a member of the homeodomain-interacting protein kinase family, specifically a serine/threonine nuclear protein kinase. HIPK2 plays versatile roles in cellular processes, including transcription regulation, cell proliferation, differentiation, and apoptosis. It interacts with various transcription factors and proteins, contributing significantly to the modulation of gene expression. Notably, HIPK2 is involved in the activation of the tumor suppressor protein p53, with p53-dependent pro-apoptotic activity, positioning HIPK2 as a potential tumor suppressor gene. Recent studies highlight its downregulation in multiple tumor types and its emerging role in cancer biology and therapy. Its expression is subject to quality control mechanisms, including autophosphorylation at serine and threonine sites. Structurally, the crystal structure of HIPK2 reveals unique conformational attributes that contribute to its functionality in different signaling pathways. HIPK2's influence extends to physiological processes, such as fibrosis and central nervous system regulation, indicating its broad biological significance and potential therapeutic applications.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: hHIPk2; Homeodomain-interacting protein kinase 2; Nuclear body-associated kinase 1; Sialophorin tail-associated nuclear serine/threonine-protein kinase
基因别名: 1110014O20Rik; B230339E18Rik; HIPK2; Nbak1; PRO0593; Stank
UniProt ID: (Human) Q9H2X6, (Mouse) Q9QZR5
Entrez Gene ID: (Human) 28996, (Mouse) 15258
RNA polymerase II activating transcription factor binding
RNA polymerase II transcription coactivator activity
transcription corepressor activity
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
SMAD binding
virion binding
nucleotide binding
DNA binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
negative regulation of transcription from RNA polymerase II promoter
eye development
transcription, DNA-templated
protein phosphorylation
DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
transforming growth factor beta receptor signaling pathway
smoothened signaling pathway
adult walking behavior
positive regulation of cell proliferation
anterior/posterior pattern specification
retina layer formation
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
modulation by virus of host morphology or physiology
neuron differentiation
erythrocyte differentiation
positive regulation of transforming growth factor beta receptor signaling pathway
negative regulation of BMP signaling pathway
PML body organization
positive regulation of protein binding
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of DNA binding
negative regulation of neuron apoptotic process
positive regulation of angiogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
embryonic camera-type eye morphogenesis
voluntary musculoskeletal movement
positive regulation of sequence-specific DNA binding transcription factor activity
regulation of cell cycle
embryonic retina morphogenesis in camera-type eye
lens induction in camera-type eye
SMAD protein signal transduction
iris morphogenesis
cellular response to hypoxia
intrinsic apoptotic signaling pathway
regulation of signal transduction by p53 class mediator
positive regulation of protein phosphorylation
regulation of transcription, DNA-templated
apoptotic process
cellular response to DNA damage stimulus
adult locomotory behavior
phosphorylation
negative regulation of nucleic acid-templated transcription
