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Invitrogen
HK2 Monoclonal Antibody (3D3)
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HK2 Antibody (MA5-15679) in ICC/IF
Immunofluorescence analysis of HK2 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HK2 Monoclonal Antibody (3D3) (Product # MA5-15679) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27... View More
产品信息
MA5-15679
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
C. elegans,
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
3D3
抗原
Purified recombinant fragment of human HK2 expressed in E. Coli.
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
ascites
内含物
0.03% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA5-15679 targets HK2 in indirect ELISA, FACS, IHC and WB applications and shows reactivity with Human samples.
The MA5-15679 immunogen is purified recombinant fragment of human HK2 expressed in E. Coli.
MA5-15679 detects HK2 which has a predicted molecular weight of approximately 102kDa.
靶标信息
In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DKFZp686M1669; Hexokinase; Hexokinase type II; Hexokinase-2; hexokinase-2 muscle; hexokinase-2, muscle; Hexokinase-B; HK II; Muscle form hexokinase; OTTHUMP00000202738
基因别名: HK2; HKII; HXK2
UniProt ID: (Human) P52789
Entrez Gene ID: (Human) 3099
cellular glucose homeostasis
response to ischemia
glycolytic process
lactation
apoptotic mitochondrial changes
glucose transport
negative regulation of mitochondrial membrane permeability
regulation of glucose import
carbohydrate phosphorylation
glucose 6-phosphate metabolic process
canonical glycolysis
establishment of protein localization to mitochondrion
maintenance of protein location in mitochondrion
positive regulation of mitophagy in response to mitochondrial depolarization
negative regulation of reactive oxygen species metabolic process
