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HLA-ABC Monoclonal Antibody (W6/32), Super Bright™ 436, eBioscience™
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HLA-ABC Antibody (62-9983-42) in Flow
Normal human peripheral blood cells were stained with Mouse IgG2a kappa Isotype Control, Super Bright 436 (Product # 62-4724) (blue histogram) or HLA-ABC Monoclonal Antibody, Super Bright 436 (purple histogram). Cells in the lymphocyte gate were used for analysis.
产品信息
62-9983-42
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
W6/32
偶联物
激发/发射光谱
413/431 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey.
Applications Reported: This W6/32 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This W6/32 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
靶标信息
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: allele B-3515; B*3509; cell surface glycoprotein; integral membrane protein; exons 2-8; glycosylation aa 86, alpha domain 1 aa 1-24, alpha domain 2 aa 25-114, alpha domain 3 aa 207-298; glycosylation aa 86, alpha domain 1 aa 25-114, alpha domain 2 aa 115-206, alpha domain 3 aa 207-298; H-2K; H-2K(d); H2-K; heavy chain; hla antigen; HLA B-1519; HLA class I antigen HLA-B; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, B alpha chain; HLA class I histocompatibility antigen, C alpha chain; HLA protein allele A25; HLA protein allele B37; HLA protein allele B51; HLA protein allele B57; HLA protein allele B7; HLA-A; HLA-A 2402; HLA-A*0201 alpha chain; human leukocyte antigen; HLA-A*0204; HLA-A*0207 alpha chain; human leukocyte antigen; HLA-A-0102 allele; HLA-A24AK; HLA-A25; HLA-B; HLA-B 1502; HLA-B*4002; HLA-B-07ZEL allele; HLA-B-1501; HLA-B-1516; HLA-B-1520 allele; HLA-B-15AOH allele; HLA-B-15ZEL allele; HLA-B-27KSH allele; HLA-B-67LAV allele; This submission reports an HLA-B-6701 variant (HLA-B-67LAV) that differs from the published B-6701 sequence, GenBank Accession Number L17005; HLA-B18; HLA-B27 peptide; HLA-B39; HLA-B41 variant; HLA-B61; HLA-Bw-39-DBU allele; HLA-C; HLA-C antigen; HLA-Cw; HLA-Cw1 protein; HLA-Cw2 protein; HLA-Cw3 protein; human leukocyte antigen; Human leukocyte antigen A; Human leukocyte antigen B; Human leukocyte antigen C; human leukocyte antigen-C alpha chain; leucocyte antigen A; leukocyte antigen class I-A; leukocyte antigen class I-B; lymphocyte antigen; major histocompatibility antigen HLA-C; major histocompatibility complex class I A; major histocompatibility HLA-Cw2.2 heavy chain; MGC184092; MGC7052; MHC 1; MHC Class 1; MHC class 1 antigen; MHC class I; MHC class I antigen heavy chain HLA-C; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-B alpha chain; MHC class I antigen HLA-B heavy chain; MHC class I antigen SHCHA; MHC class I molecule; MHC class I-like alpha chain membrane-bound type; MHC HLA-A cell surface antigen; MHC HLA-A10-alpha-2 chain precursor; MHC HLA-A2.4a chain; MHC HLA-A24 cell surface glycoprotein; MHC HLA-ABC; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC HLA-B44; MHC HLA-B7 heavy chain precursor; MHC HLA-Bw46; MHC HLA-C-alpha-2 chain precursor; MHC I HLA; MHC1; similar to Homo sapiens MHC HLA-B39 mRNA with GenBank Accession Number L42024.1; subtype B*3508 differs at residue 156 (Leu->Arg) with subtype B*3501; unnamed protein product
基因别名: AS; B-4901; D6S204; HLA-A; HLA-B; HLA-C; HLA-JY3; HLAA; HLAB; HLAC; HLC-C; MHC; PSORS1
UniProt ID: (Human) P04439, (Human) P01889, (Human) P10321
Entrez Gene ID: (Human) 3105, (Human) 3106, (Human) 3107
T cell mediated cytotoxicity
positive regulation of T cell mediated cytotoxicity
adaptive immune response
immune system process
T cell mediated cytotoxicity directed against tumor cell target
antigen processing and presentation of peptide antigen via MHC class I
antigen processing and presentation of endogenous peptide antigen via MHC class Ib
antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent
antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent
peptide antigen assembly with MHC class I protein complex
positive regulation of T cell cytokine production
immune response
detection of bacterium
antibacterial humoral response
antigen processing and presentation
antigen processing and presentation of endogenous peptide antigen via MHC class I
positive regulation of interferon-gamma production
CD8-positive, alpha-beta T cell activation
protection from natural killer cell mediated cytotoxicity
antigen processing and presentation of exogenous peptide antigen via MHC class I
innate immune response
defense response to Gram-positive bacterium
T cell receptor signaling pathway
positive regulation of CD8-positive, alpha-beta T cell proliferation
positive regulation of memory T cell activation
positive regulation of CD8-positive, alpha-beta T cell activation
signal transduction
positive regulation of natural killer cell mediated cytotoxicity
regulation of T cell anergy
defense response
regulation of interleukin-12 production
regulation of interleukin-6 production
regulation of dendritic cell differentiation
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