Invitrogen

CD3e Monoclonal Antibody (500A2), Alexa Fluor™ 647

Name: CD3e Monoclonal Antibody (500A2), Alexa Fluor\u2122 647
Brand: Invitrogen
SKU: HM3421

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6 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

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CD3e Antibody (HM3421) in Flow

Scientific reports 2015 - Figure 1 Representative flow cytometric histographs for CCR2 expression in leukocyte subsets as detected by CCR2 reporter protein RFP. Cell suspensions of the peripheral blood, BM, spleen and cervical LNs from the CCR2 RFP reporter mice (CCR2-RFP +/- ) and age-and sex-matched non-transgenic WT C57BL/6J mice were stained with differential subset-specific mAbs. In flow cytometric analyses, subset identificati... View More

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Immunogen Image CD3e Monoclonal Antibody (500A2), Alexa Fluor™ 647

产品信息

HM3421

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

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流式细胞分析 (Flow)

0.1-0.25 µg / 1x10^6 cells

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中和实验 (Neu)

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产品规格

种属反应

Mouse

已发表种属

C. elegans, Mouse

宿主/亚型

Syrian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

500A2

抗原

C6VL-BS cell lysate

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偶联物

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

激发/发射光谱

650/671 nm 查看光谱

形式

Liquid

浓度

0.1 mg/mL

纯化类型

purified

保存液

PBS with BSA

内含物

0.1% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2536582

靶标信息

The CD3 complex, composed of gamma, delta, epsilon, and zeta subunits, is essential for the assembly, trafficking, and surface expression of the T cell receptor (TCR) complex. These subunits are structurally related members of the immunoglobulin superfamily and are encoded by closely linked genes on human chromosome 11. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, but not on B or NK cells. The CD3 subunits play a crucial role in transducing antigen-recognition signals into the cytoplasm of T cells. The cytoplasmic tails of CD3 subunits contain a double tyrosine-based motif that associates with cytoplasmic signal transduction molecules, mediating T cell activation through the TCR. Crosslinking of the TCR initiates intracellular biochemical pathways that result in cellular activation, proliferation, and potentially growth arrest and cell survival. CD3 is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes, and Purkinje cells in the cerebellum. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Defects in the CD3 gene are associated with CD3 immunodeficiency, highlighting its importance in immune function and regulation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 TCR complex; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

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基因别名: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz