Invitrogen

HMGA2 Polyclonal Antibody

Advanced Verification
2 Published Figures
3 References
View all (3) HMGA2 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

PA5-21320

Applications
Tested Dilution
Publications

Western Blot (WB)

1:5,000-1:50,000
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

Immunoprecipitation (IP)

1:100-1:500
-

ChIP assay (ChIP)

Assay-dependent
-

Affinity Purification (AP)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

C. elegans, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGA2. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11155799

Product Specific Information

Recommended positive controls: A431, HeLa, HepG2, HCT116.

Predicted reactivity: Mouse (95%), Rat (92%), Zebrafish (85%), Xenopus laevis (80%), Chicken (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

HMGA2, previously known as HMGI-C, belongs to the group of high mobility chromosomal proteins that have the AT-hook DNA-binding motif. It is highly and ubiquitously expressed during embryogenesis. Chromosomal rearrangements of the HMGA2 gene and overexpression are frequently associated with benign and malignant tumors, respectively. Human and mouse HMGA2 shares 95% amino acid sequence identity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: High mobility group AT-hook protein 2; High mobility group protein HMGI-C; high mobility group protein I, isoform C; high-mobility group (nonhistone chromosomal) protein isoform I-C; High-mobility group protein HMGI-C; non-histone chromosomal architectural protein HMGI-C

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Gene Aliases: 9430083A20Rik; BABL; HMGA2; HMGI-C; HMGIC; LIPO; pg; pygmy; STQTL9

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UniProt ID: (Human) P52926, (Mouse) P52927

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Entrez Gene ID: (Human) 8091, (Mouse) 15364, (Rat) 84017

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Function(s)
regulatory region DNA binding transcription factor activity, transcription factor binding core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding AT DNA binding DNA-(apurinic or apyrimidinic site) lyase activity DNA-dependent protein kinase activity protein binding transcription factor binding DNA binding, bending nucleosomal DNA binding cAMP response element binding MH2 domain binding MH1 domain binding SMAD binding 5'-deoxyribose-5-phosphate lyase activity C2H2 zinc finger domain binding enzyme binding transcription factor activity, sequence-specific DNA binding endodeoxyribonuclease
Process(es)
negative regulation of transcription from RNA polymerase II promoter epithelial to mesenchymal transition chondrocyte differentiation mesodermal-endodermal cell signaling base-excision repair chromatin organization regulation of transcription, DNA-templated transcription from RNA polymerase II promoter mitotic nuclear division mitotic G2 DNA damage checkpoint multicellular organism development response to virus regulation of cell cycle process positive regulation of gene expression chromosome condensation chromosome breakage heterochromatin assembly histone H2A-S139 phosphorylation senescence-associated heterochromatin focus assembly endodermal cell differentiation chondrocyte proliferation regulation of growth DNA damage response, detection of DNA damage positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of DNA binding negative regulation by host of viral transcription fat cell differentiation negative regulation of single stranded viral RNA replication via double stranded DNA intermediate negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter mesodermal cell differentiation mesenchymal cell differentiation stem cell differentiation cell division positive regulation of cell cycle arrest oncogene-induced cell senescence regulation of stem cell population maintenance positive regulation of stem cell proliferation positive regulation of transcription regulatory region DNA binding positive regulation of cellular response to X-ray positive regulation of cellular senescence positive regulation of response to DNA damage stimulus negative regulation of double-strand break repair via nonhomologous end joining regulation of cellular response to drug transcription, DNA-templated cell cycle signal transduction spermatogenesis cell proliferation positive regulation of cell proliferation male gonad development cell proliferation in forebrain pituitary gland development adrenal gland development negative regulation of intracellular steroid hormone receptor signaling pathway somatic stem cell population maintenance positive regulation of multicellular organism growth negative regulation of JAK-STAT cascade negative regulation of astrocyte differentiation positive regulation of sequence-specific DNA binding transcription factor activity meiotic cell cycle regulation of growth hormone secretion adipose tissue development fat pad development regulation of peptide hormone secretion nucleic acid phosphodiester bond hydrolysis DNA catabolic process, endonucleolytic
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