Invitrogen

HMGB1 Monoclonal Antibody (GT348)

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问题与答案
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说明书
实验方案
问题与答案
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产品信息

MA5-17278

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:500-1:3,000
查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

-
查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000
-

免疫组化（冰冻） (IHC (F))

1:100-1:1,000
-

免疫细胞化学 (ICC/IF)

1:100-1:1,000
查看1篇已发表文章 1篇已发表文章

亲和纯化 (AP)

-
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产品规格

种属反应

Human, Mouse, Rat

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

GT348

抗原

Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.88 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2538744

产品详细信息

Recommended positive controls: 293T, A431, HepG2, Neuro2A, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2, mouse brain, rat brain.

Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (93%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

High-mobility group box 1 protein, previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. Moreover, HMGB1 is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Amphoterin; Amphoterin antibody; DKFZp686A04236; Heparin-binding protein p30; high mobility group 1; High mobility group protein 1; High mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; HMG-1; HMG3 antibody; hmgb 1; hmgb-1; RP11-550P23.1; Sulfoglucuronyl carbohydrate binding protein

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基因别名: Ac2-008; amphoterin; DEF; HMG-1; HMG1; HMG3; HMGB1; p30; SBP-1

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UniProt ID: (Human) P09429, (Rat) P63159, (Mouse) P63158

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Entrez Gene ID: (Human) 3146, (Rat) 25459, (Mouse) 15289

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功能
four-way junction DNA binding bubble DNA binding lipopolysaccharide binding phosphatidylserine binding damaged DNA binding double-stranded DNA binding single-stranded DNA binding transcription factor activity, sequence-specific DNA binding double-stranded RNA binding single-stranded RNA binding cytokine activity protein binding transcription factor binding DNA binding, bending calcium-dependent protein kinase regulator activity lyase activity C-X-C chemokine binding protein kinase activator activity chemoattractant activity poly(A) RNA binding RAGE receptor binding DNA polymerase binding repressing transcription factor binding supercoiled DNA binding open form four-way junction DNA binding crossed form four-way junction DNA binding DNA binding bent DNA binding chromatin binding 5S rRNA binding heparin binding peptide binding protein dimerization activity glycolipid binding
参与通路
negative regulation of transcription from RNA polymerase II promoter eye development myeloid dendritic cell activation endothelial cell proliferation activation of innate immune response plasmacytoid dendritic cell activation macrophage activation involved in immune response dendritic cell chemotaxis inflammatory response to antigenic stimulus regulation of tolerance induction regulation of T cell mediated immune response to tumor cell DNA topological change base-excision repair apoptotic DNA fragmentation DNA recombination regulation of transcription from RNA polymerase II promoter autophagy inflammatory response positive regulation of cytosolic calcium ion concentration regulation of autophagy negative regulation of RNA polymerase II transcriptional preinitiation complex assembly lung development neuron projection development chromatin assembly regulation of restriction endodeoxyribonuclease activity activation of protein kinase activity DNA geometric change positive regulation of mismatch repair negative regulation of interferon-gamma production positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of tumor necrosis factor production V(D)J recombination positive regulation of toll-like receptor 2 signaling pathway positive regulation of toll-like receptor 4 signaling pathway positive regulation of toll-like receptor 9 signaling pathway T-helper 1 cell activation endothelial cell chemotaxis positive regulation of activated T cell proliferation positive regulation of apoptotic process apoptotic cell clearance positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of CD4-positive, alpha-beta T cell differentiation positive regulation of DNA binding positive regulation of MAPK cascade negative regulation of blood vessel endothelial cell migration T-helper 1 cell differentiation innate immune response positive regulation of myeloid cell differentiation positive regulation of glycogen catabolic process positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of interleukin-1 secretion positive regulation of interleukin-1 beta secretion positive chemotaxis DNA ligation involved in DNA repair positive regulation of DNA ligation response to glucocorticoid positive regulation of ERK1 and ERK2 cascade positive regulation of monocyte chemotaxis positive regulation of wound healing neutrophil clearance positive regulation of NIK/NF-kappaB signaling positive regulation of sprouting angiogenesis tumor necrosis factor secretion negative regulation of apoptotic cell clearance positive regulation of interleukin-6 secretion regulation of nucleotide-excision repair positive regulation of dendritic cell differentiation cell morphogenesis positive regulation of protein phosphorylation positive regulation of mesenchymal cell proliferation base-excision repair, DNA ligation chromatin remodeling regulation of transcription, DNA-templated chemotaxis nervous system development circadian rhythm negative regulation of DNA replication positive regulation of cell proliferation response to heat response to glucose positive regulation of cell death positive regulation of neuron projection development positive regulation of smooth muscle cell migration positive regulation of cell migration actin cytoskeleton reorganization response to lipopolysaccharide response to insulin positive regulation of myeloid cell apoptotic process response to interferon-gamma response to drug positive regulation of myoblast differentiation positive regulation of protein kinase activity positive regulation of mitotic cell cycle regulation of inflammatory response male-specific defense response to bacterium induction of positive chemotaxis myoblast proliferation cellular response to interleukin-1
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