Invitrogen

HMGCR Polyclonal Antibody

Name: HMGCR Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-37367

Advanced Verification

12 Published Figures

14 References

View all (15) HMGCR antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

HMGCR Antibody in Immunocytochemistry (ICC/IF)

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HMGCR Antibody (PA5-37367) in ICC/IF

Immunofluorescence analysis of HMGCR was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with HMGCR Polyclonal Antibody (Product # PA5-37367) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32... View More

HMGCR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HMGCR Antibody in Immunohistochemistry (IHC)

HMGCR Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

HMGCR Antibody — Fig. 6 a , b , Representative images and quantification of migrated or invaded LNCaP cells in the migration and invasion assay. LNCaP cells were transfected with the indicated control or target siRNAs against PML or/and SREBP-1 ( a ) or were pretreated with DMSO, 10 mug/ml TOFA or 10 muM simvastatin ( b ) for 48 h, then subjected to migration (24 h) or invasion (48 h) assays. Scale bars, 50 mum. c - g , Gross anatomy of representative Pten pc-/- ; Pml pc-/- tumors ( c ), quantification of tumor weight ( d ) and the incidence of metastasis ( e ) and immunoblot ( f ) and IHC ( g ) analyses of tumors from Pten pc-/- ; Pml pc-/- mice after treatment with vehicle or 15 mg/kg fatostatin for two months. Scale bars, 5 mm ( c ) and 50 mum ( g ). Arrowheads in g indicate apoptotic cells. In a and b , the results of one representative experiment are shown ( n = 5 experiments). Data are from three independent cultures (4 fields per insert). In d , n = 6 mice per treatment. Data shown in a , b and d are mean +- s.e.m. Student's t test (two tailed) was used to determine significance. Uncropped images for f are shown in Supplementary Fig. 7 . Numbers to left of blot indicate molecular weight (kDa). {TM}

Immunogen image

Immunogen Image HMGCR Polyclonal Antibody

Product Details

PA5-37367

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

1:50-1:200

View 1 publication 1 publication

Affinity Purification (AP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

C. elegans, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant full length human HMGCR

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2554032

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 97 kDa.

Purity is >95% by SDS-PAGE.

Target Information

3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR) is a key enzyme in the mevalonate pathway, which is critical for the biosynthesis of cholesterol and other isoprenoids. HMGCR catalyzes the conversion of HMG-CoA to mevalonate, a rate-limiting step in the production of cholesterol. Due to its central role in cholesterol synthesis, HMGCR is a primary target for statin drugs, which are used to lower cholesterol levels and reduce the risk of cardiovascular disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 3-hydroxy-3-methylglutaryl CoA reductase (NADPH); 3-hydroxy-3-methylglutaryl-CoA reductase; 3-hydroxy-3-methylglutaryl-coenzyme A reductase; HMDH; HMG CoA reductase; HMG CoAR; HMG-CoA reductase; hydroxymethylglutaryl-CoA reductase; MGC103269

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Gene Aliases: 3H3M; HMG-CoAR; HMGCR; LDLCQ3; Red

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UniProt ID: (Human) P04035, (Mouse) Q5U4I2, (Rat) P51639

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Entrez Gene ID: (Human) 3156, (Mouse) 15357, (Rat) 25675

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Function(s)

hydroxymethylglutaryl-CoA reductase (NADPH) activity protein binding hydroxymethylglutaryl-CoA reductase activity protein homodimerization activity coenzyme binding protein phosphatase 2A binding NADPH binding oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor NADP binding oxidoreductase reductase metabolite interconversion enzyme

Process(es)

cholesterol biosynthetic process ubiquinone metabolic process aging response to nutrient isoprenoid biosynthetic process visual learning negative regulation of striated muscle cell apoptotic process positive regulation of cardiac muscle cell apoptotic process coenzyme A metabolic process positive regulation of stress-activated MAPK cascade negative regulation of MAP kinase activity myoblast differentiation response to ethanol negative regulation of vasodilation positive regulation of skeletal muscle tissue development positive regulation of smooth muscle cell proliferation protein tetramerization oxidation-reduction process negative regulation of wound healing negative regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of ERK1 and ERK2 cascade lipid metabolic process steroid biosynthetic process steroid metabolic process cholesterol metabolic process positive regulation of cell proliferation embryo development sterol biosynthetic process negative regulation of apoptotic process farnesyl diphosphate biosynthetic process, mevalonate pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HMGCR Polyclonal Antibody

HMGCR Antibody (PA5-37367) in ICC/IF

Product Details

PA5-37367

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Affinity Purification (AP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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