Invitrogen

HMOX1 Monoclonal Antibody (GT17811)

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抗体检测数据 (4)

HMOX1 Antibody in Immunocytochemistry (ICC/IF)

HMOX1 Antibody in Immunocytochemistry (ICC/IF)

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HMOX1 Antibody (MA5-31558) in ICC/IF

Immunofluorescence analysis of HMOX1 was performed using 80% confluent log phase HeLa cobalt chloride treated (500 micromolar for 24h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with HMOX1 Monoclonal Antibody (GT17811) (Product # MA5-31558) at 1:100 dilution in 0.1% BSA, incubated at 4 degrees celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Sec... View More

HMOX1 Antibody in Immunocytochemistry (ICC/IF)

HMOX1 Antibody in Immunocytochemistry (ICC/IF)

HMOX1 Antibody in Western Blot (WB)

HMOX1 Antibody in ELISA (ELISA)

Product Image Gallery

HMOX1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of HMOX1 was performed using 80% confluent log phase HeLa cobalt chloride treated (500 micromolar for 24h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with HMOX1 Monoclonal Antibody (GT17811) (Product # MA5-31558) at 1:100 dilution in 0.1% BSA, incubated at 4 degrees celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766, 1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing endoplasmic reticulum and nuclear localization. Panel e represents the untreated cells showing no HMOX1 expression. Panel f represents control cells with no primary antibody to assess the background. The images were captured at 60X magnification.

HMOX1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of HMOX1 was performed in Mock and treated HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HMOX1 Monoclonal Antibody (GT17811) (Product # MA5-31558) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

HMOX1 Antibody in Western Blot (WB) — Western blot analysis of HMOX1 in untreated (–) and treated (+) HeLa whole cell extract using HMOX1 monoclonal antibody (Product # MA5-31558) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 15% SDS-PAGE.

HMOX1 Antibody in ELISA (ELISA) — Sandwich ELISA detection of recombinant full-length Heme Oxygenase 1 protein using HMOX1 Monoclonal Antibody (GT17811) (Product # MA5-31558) as capture antibody at concentration of 5 µg/mL and HMOX1 Polyclonal Antibody (Product # PA5-27338) as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) was diluted at 1:10,000 and used to detect the primary antibody.

产品信息

MA5-31558

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

-

免疫细胞化学 (ICC/IF)

1:100-1:1,000

-

酶联免疫吸附实验 (ELISA)

Assay-dependent

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

GT17811

抗原

Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2787186

产品详细信息

Keep as concentrated solution.

Predicted reactivity: Mouse (81%), Rat (80%), Dog (85%), Pig (82%), Bovine (83%).

Positive Control: HeLa(500 µM CoCl2 treatment for 24 hr).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; Heme oxygenase 1; HO-1

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基因别名: bK286B10; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32

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UniProt ID: (Human) P09601

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Entrez Gene ID: (Human) 3162

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heme oxygenase (decyclizing) activity phospholipase D activity signal transducer activity protein binding enzyme binding heme binding protein homodimerization activity metal ion binding oxygenase

参与通路

angiogenesis endothelial cell proliferation wound healing involved in inflammatory response negative regulation of leukocyte migration heme oxidation cellular iron ion homeostasis response to oxidative stress small GTPase mediated signal transduction excretion regulation of blood pressure cell death intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of muscle cell apoptotic process smooth muscle hyperplasia cellular response to nutrient negative regulation of mast cell cytokine production erythrocyte homeostasis low-density lipoprotein particle clearance regulation of transcription from RNA polymerase II promoter in response to iron cellular response to heat response to nicotine intracellular signal transduction heme catabolic process response to hydrogen peroxide positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of mast cell degranulation negative regulation of DNA binding negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of neuron apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress response to estrogen positive regulation of chemokine biosynthetic process regulation of angiogenesis positive regulation of angiogenesis positive regulation of vasodilation positive regulation of smooth muscle cell proliferation negative regulation of smooth muscle cell proliferation regulation of sequence-specific DNA binding transcription factor activity protein homooligomerization iron ion homeostasis cellular response to arsenic-containing substance cellular response to cadmium ion cellular response to hypoxia negative regulation of extrinsic apoptotic signaling pathway via death domain receptors