Invitrogen

HMOX1 Monoclonal Antibody (HO-1-1)

Name: HMOX1 Monoclonal Antibody (HO-1-1)
Brand: Invitrogen
SKU: MA1-112

高级验证

21个已发表图片

38 篇参考文献

查看其他34个HO-1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

HMOX1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 32

HMOX1 Antibody (MA1-112) in ICC/IF

Immunofluorescent analysis of Heme Oxygenase-1 (HO-1, green) in HeLa cells either left untreated (left panel) or treated with 0.5mM copper sulfate (right panel) for 24 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an HO-1 monoclonal antibody (Product # MA1-112) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight ... View More

HMOX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HMOX1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

HMOX1 Antibody in Immunohistochemistry (IHC)

HMOX1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

HMOX1 Antibody — Modulation of target protein expression by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of HO-1 monoclonal antibody (Product # MA1-112) shows induced expression of HO-1 in the HeLa cells upon treatment with copper sulphate. {TM}

Immunogen image

Immunogen Image HMOX1 Monoclonal Antibody (HO-1-1)

产品信息

MA1-112

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看20篇已发表文章 20篇已发表文章

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:200

查看6篇已发表文章 6篇已发表文章

流式细胞分析 (Flow)

10 µg/mL

查看1篇已发表文章 1篇已发表文章

亲和纯化 (AP)

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Bovine, Dog, Human, Mouse, Rat

已发表种属

C. elegans, Guinea pig, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

HO-1-1

抗原

Synthetic peptide corresponding to the sequence near the N-terminus (amino acids 1-30) of human Heme Oxygenase 1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2536823

产品详细信息

This antibody detects an ~32 kDa protein corresponding to the apparent molecular mass of HO-1 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine and dog origins. This antibody does not cross-react with HO-2.

This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

靶标信息

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; Heme oxygenase 1; hemoxygenase; HO-1; HSP32; P32 protein

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基因别名: bK286B10; D8Wsu38e; Hemox; Heox; HEOXG; Hmox; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32