Invitrogen

HO-1 Polyclonal Antibody

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1 篇参考文献

查看其他34个HO-1抗体

问题与答案

问题与答案

抗体检测数据 (7)
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HO-1 Antibody in Immunocytochemistry (ICC/IF)

HO-1 Antibody in Immunocytochemistry (ICC/IF)

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HO-1 Antibody (PA5-119658) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of Heme Oxygenase 1 (HO-1) in HepG2 cells (green) using HO-1 Polyclonal Antibody (Product # PA5-119658). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

HO-1 Antibody in Immunocytochemistry (ICC/IF)

HO-1 Antibody in Immunocytochemistry (ICC/IF)

HO-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HO-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HO-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HO-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HO-1 Antibody in Western Blot (WB)

HO-1 Antibody in Western Blot (WB)

HO-1 Antibody in Western Blot (WB)

HO-1 Antibody in Western Blot (WB)

Product Image Gallery

HO-1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of Heme Oxygenase 1 (HO-1) in HepG2 cells (green) using HO-1 Polyclonal Antibody (Product # PA5-119658). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

HO-1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of Heme Oxygenase 1 (HO-1) in 293T cells (green) using HO-1 Polyclonal Antibody (Product # PA5-119658). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

HO-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse small intestine tissue using HO-1 Polyclonal Antibody (Product # PA5-119658). Counter stained with hematoxylin.

HO-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of paraffin-embedded human small intestine tissue using HO-1 Polyclonal Antibody (Product # PA5-119658). Counter stained with hematoxylin.

HO-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of paraffin-embedded human placenta tissue using HO-1 Polyclonal Antibody (Product # PA5-119658). Counter stained with hematoxylin.

HO-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of paraffin-embedded human spleen tissue using HO-1 Polyclonal Antibody (Product # PA5-119658). Counter stained with hematoxylin.

HO-1 Antibody in Western Blot (WB) — Western blot analysis of Heme Oxygenase 1 (HO-1) on mouse spleen tissue lysate using HO-1 Polyclonal Antibody (Product # PA5-119658).

HO-1 Antibody in Western Blot (WB) — Figure 1. NEAT1 and Apelin are dysregulated in mice with T2DM exposed to IH . KKAy mice were divided into the T2DM + IH group (mice exposed to intermittent hypoxia) and the T2DM group (mice exposed to intermittent normoxic air). (a) fasting blood glucose was measured using a glucose meter in each mouse. (b) qRT-PCR was performed to detect NEAT1 and Apelin expression in the aortic vascular tissues of each mouse. (c) protein expression levels of Apelin, Nrf2, and HO-1 were examined using western blot in the aortic vascular tissues of each mouse. (d and e) SOD activity and GSH production were assessed using commercial assay kits in the aortic vascular tissues of each mouse. (f) ELISA was performed to analyze the levels of TNF-alpha, IL-6, IL-1beta, and IL-8 in the serum of each mouse. ** p < 0.01, *** p < 0.001.

HO-1 Antibody in Western Blot (WB) — Figure 6. NEAT1 silencing protected against HG- and IH-induced HMEC-1 cell injury by modulating Apelin . HMEC-1 cells were divided into the control, HG-IH, HG-IH + sh-NEAT1, HG-IH + sh-NEAT1 + si-NC, and HG-IH + sh-NEAT1 + si-Apelin groups. NEAT1 expression was assessed using qRT-PCR (a). Apelin expression was detected using qRT-PCR and western blot (b and c). Cell viability was analysed using CCK-8 assay (d). Cell apoptosis was analysed using flow cytometry (e). TNF-alpha, IL-1beta, IL-6, and IL-8 levels were detected using ELISA (f). ROS level, MDA level, SOD activity, and GSH production were assessed using commercial assay kits (g and h). Nrf2 and HO-1 protein expression was detected using western blot (i). * p < 0.05, ** p < 0.01, *** p < 0.001.

HO-1 Antibody in Western Blot (WB) — Figure 5. Apelin overexpression protected against HG- and IH-induced HMEC-1 cell injury by activating the Nrf2/HO-1 signalling pathway . HMEC-1 cells were divided into the control, HG-IH, HG-IH + oe-NC, and HG-IH + oe-Apelin groups. Apelin expression was analysed using qRT-PCR and western blot (a and g). Cell viability was detected using CCK-8 assay (c). Cell apoptosis was analysed using flow cytometry (d). TNF-alpha, IL-1beta, IL-6, and IL-8 levels were analysed using ELISA (e). ROS level, MDA level, SOD activity, and GSH production were assessed using commercial assay kits (f and g). Nrf2 and HO-1 protein expression was detected using western blot (h). * p < 0.05, ** p < 0.01, *** p < 0.001.

Immunogen image

HO-1 Polyclonal Antibody

Immunogen Image HO-1 Polyclonal Antibody

产品信息

PA5-119658

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

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免疫组化（石蜡） (IHC (P))

1:2,000

-

免疫细胞化学 (ICC/IF)

1:500

-

流式细胞分析 (Flow)

1:1,000

-

产品规格

种属反应

Human, Mouse

已发表种属

C. elegans

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide of N-terminal human Heme Oxygenase 1 (HO-1)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Positive Control: Mouse spleen tissue lysate, 293T, HepG2, human spleen tissue, human placenta tissue, human small intestine tissue, mouse small intestine tissue.

Subcellular Location: Endoplasmic reticulum.

靶标信息

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; Heme oxygenase 1; hemoxygenase; HO-1; P32 protein

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基因别名: bK286B10; D8Wsu38e; Hemox; Hmox; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32

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UniProt ID: (Human) P09601, (Mouse) P14901

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Entrez Gene ID: (Human) 3162, (Mouse) 15368

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heme oxygenase (decyclizing) activity phospholipase D activity signal transducer activity protein binding enzyme binding heme binding protein homodimerization activity metal ion binding oxidoreductase activity oxygenase

angiogenesis endothelial cell proliferation wound healing involved in inflammatory response negative regulation of leukocyte migration heme oxidation cellular iron ion homeostasis response to oxidative stress small GTPase mediated signal transduction excretion regulation of blood pressure cell death intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of muscle cell apoptotic process smooth muscle hyperplasia cellular response to nutrient negative regulation of mast cell cytokine production erythrocyte homeostasis low-density lipoprotein particle clearance regulation of transcription from RNA polymerase II promoter in response to iron cellular response to heat response to nicotine intracellular signal transduction heme catabolic process response to hydrogen peroxide positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of mast cell degranulation negative regulation of DNA binding negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of neuron apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress response to estrogen positive regulation of chemokine biosynthetic process regulation of angiogenesis positive regulation of angiogenesis positive regulation of vasodilation positive regulation of smooth muscle cell proliferation negative regulation of smooth muscle cell proliferation regulation of sequence-specific DNA binding transcription factor activity protein homooligomerization iron ion homeostasis cellular response to arsenic-containing substance cellular response to cadmium ion cellular response to hypoxia negative regulation of extrinsic apoptotic signaling pathway via death domain receptors apoptotic process negative regulation of cell proliferation heme metabolic process oxidation-reduction process