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Invitrogen
HRV3C Polyclonal Antibody
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HRV3C Antibody (PA1-118) in WB
Western blot analysis of HRV3c was performed by loading 1 µg of a control protein, either uncleaved (lane 1) or cleaved (lane 2) using the Pierce Human Rhinovirus 3C Protease (HRV 3C) (Product # 88946) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRV3c polyclonal antibody (Product # PA1-118) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti... View More
产品信息
PA1-118
产品规格
种属反应
Virus
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide recognizing the cleavage site of HRV3c protease
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA, 30% glycerol
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1118 has been used successfully in Western Blot to detect HRV3C cleavage site (Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro).
PA1118 has been used in Immunoprecipitation of an HRV3C cleavage site-containing protein from a whole Hela cell lysate.
靶标信息
Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. It cleaves between Gln and Gly (independent of Pro). (HRV) 3C Protease is used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is typically dual tagged for easy removal from the sample after cleavage.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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