Invitrogen

HSP27 Recombinant Mouse Monoclonal Antibody (STRSN), Alexa Fluor™ Plus 488

Name: HSP27 Recombinant Mouse Monoclonal Antibody (STRSN), Alexa Fluor\u2122 Plus 488
Brand: Invitrogen
SKU: 740230MP488

高级验证

查看其他74个HSP27抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (4)
高级验证 (2)

HSP27 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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HSP27 Antibody (740230MP488) in IHC (P)

Immunohistochemical analysis of HSP27 was performed using formalin-fixed paraffin-embedded human prostate adenocarcinoma tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for... View More

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HSP27 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of HSP27 was observed in HeLa and K-562 which is reported to be positive but not in HT-1080 and Jurkat using HSP27 Recombinant Mouse Monoclonal Antibody (STRSN), Alexa Fluor™ Plus 488 (Product # 740230MP488) in western blot. {RE}

产品信息

740230MP488

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200

免疫组化（石蜡） (IHC (P))

1:50

流式细胞分析 (Flow)

0.15 µg/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

STRSN

偶联物

Alexa Fluor™ Plus 488 Alexa Fluor™ Plus 488 Alexa Fluor™ Plus 488

激发/发射光谱

493/518 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

proprietary buffer, pH 6.3-7.3

内含物

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Wet ice

产品详细信息

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 28 kDa heat shock protein; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; Heat shock protein beta-1; Heat shock protein family B member 1; HS; HSP; HSP 27; HspB1; Phospho-HSP 27; SRP27; Stress-responsive protein 27

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基因别名: CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; HSPB1; SRP27

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UniProt ID: (Human) P04792

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Entrez Gene ID: (Human) 3315

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功能

protein kinase C binding protein binding protein kinase C inhibitor activity protein kinase binding identical protein binding ubiquitin binding poly(A) RNA binding

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