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Invitrogen
HSP60 Polyclonal Antibody
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HSP60 Antibody (PA5-34760) in ICC/IF
Immunofluorescence analysis of HSP60 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSP60 Polyclonal Antibody (Product # PA5-34760) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2703... View More
产品信息
PA5-34760
应用
建议稀释比
已发表文章
产品规格
种属反应
Hamster,
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the center region of human HSP60. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.3 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol, 1% BSA
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, rat brain.
Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (85%), Xenopus laevis (91%), Pig (99%), Chicken (94%), Rhesus Monkey (97%), Chimpanzee (99%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 60 kDa chaperonin; 60 kDa heat shock protein; 60 kDa heat shock protein, mitochondrial; Chaperonin 60; CPN60; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1; heat shock 60kDa protein 1 (chaperonin); Heat shock protein; heat shock protein 1 (chaperonin); Heat shock protein 60; heat shock protein 60 (liver); heat shock protein 65; Heat shock protein family D member 1; heat shock protein, 60 kDa; HSP; HSP-60; HSP-65; Hsp60s1; HuCHA60; Mitochondrial matrix protein P1; P1 protein; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1
基因别名: 60kDa; CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HSPD1; Hspd1-30p; HuCHA60; I79_011398; SPG13
UniProt ID: (Human) P10809, (Chinese hamster) P18687, (Mouse) P63038, (Rat) P63039
Entrez Gene ID: (Human) 3329, (Chinese hamster) 100689473, (Mouse) 15510, (Rat) 63868
lipopolysaccharide binding
p53 binding
DNA replication origin binding
single-stranded DNA binding
double-stranded RNA binding
protein binding
ATP binding
ATPase activity
ubiquitin protein ligase binding
poly(A) RNA binding
unfolded protein binding
chaperone binding
nucleotide binding
protease binding
protein complex binding
insulin binding
protein heterodimerization activity
misfolded protein binding
detection of misfolded protein
B cell cytokine production
MyD88-dependent toll-like receptor signaling pathway
positive regulation of T cell mediated immune response to tumor cell
protein folding
activation of cysteine-type endopeptidase activity involved in apoptotic process
response to unfolded protein
positive regulation of interferon-alpha production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
protein refolding
B cell proliferation
T cell activation
B cell activation
positive regulation of macrophage activation
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
isotype switching to IgG isotypes
positive regulation of inflammatory response
protein stabilization
positive regulation of T cell activation
chaperone mediated protein folding requiring cofactor
'de novo' protein folding
response to cold
viral process
chaperone-mediated protein complex assembly
protein maturation
response to hypoxia
response to heat
response to organic substance
response to organic cyclic compound
response to lipopolysaccharide
response to ATP
response to cocaine
response to drug
response to hydrogen peroxide
response to estrogen
