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Invitrogen
HSP70 Monoclonal Antibody (C92F3A-5)
高级验证
This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.
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HSP70 Antibody (MA1-10889) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of HEK293T (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3), A-431 (Lane 4) and MCF7 (Lane 5). The blot was probed with Anti-HSP70 Monoclonal Antibody (C92F3A-5) (Product # MA1-10889, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 70 kDa band corresponding to HSP70 was observed across the cell lines tested.
产品信息
MA1-10889
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Many,
Mouse,
Rat
已发表种属
Dog,
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
C92F3A-5
抗原
Human Hsp70 purified from HeLa cells.
偶联物
形式
Liquid
浓度
0.7 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.1% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_906420
产品详细信息
MA1-10889 detects Heat Shock Protein 70 in human, mouse, rat, rabbit, monkey, guinea pig, hamster, chicken, bovine, canine, ovine, porcine, fish, Drosophila and C. elegans samples and does not cross-react with Hsc70.
MA1-10889 has been successfully used in Western blot, immunohistochemistry, immunoprecipitation, ELISA and flow cytometry procedures. By Western blot, MA1-10889 is specific for the ~70 kDa Heat Shock Protein 70 protein.
MA1-10889 immunogen is human Hsp70 purified from HeLa cells, corresponding to amino acids 436-503.
靶标信息
HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 68 kDa heat shock protein; 70 kilodalton heat shock protein; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein Li 5a; heat shock 10kDa protein 1-like; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; Heat shock 70 kDa protein 4; Heat shock 70 kDa-like protein 1; Heat shock 70-related protein APG-2; heat shock 70kD protein 1A; heat shock 70kD protein 1B; heat shock 70kD protein 2; heat shock 70kD protein 4; heat shock 70kD protein-like 1; heat shock 70kDa protein 1-like; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; heat shock 70kDa protein 2; heat shock 70kDa protein 4; Heat shock protein; heat shock protein 70-1; Heat shock protein 70-1l; heat shock protein 70.1; Heat shock protein 70.2; heat shock protein alpha 2; heat shock protein cognate 70, testis; Heat shock protein family A member 1A; Heat shock protein family A member 1B; Heat shock protein family A member 1L; Heat shock protein family A member 2; Heat shock protein family H member 2; heat shock protein, 110 kDa; heat shock protein, 70 kDa 1; heat shock protein, 70 kDa 2; heat shock protein, 70 kDa 3; heat shock-induced protein; Heat shock-related 70 kDa protein 2; heat shock-related protein hsc70t; HEL S 103; HSP; Hsp68; HSP70 1; HSP70 1B; HSP70 2; HSP70 AceyltK77; hsp70 RY; HSP70-1; HSP70-1/HSP70-2; HSP70-2; HSP70-Hom; HSP70.1; HSP70.1/2; HSP70.1/HSP70.2; HSP70.3; HSP70RY; HSPA2; HSPA4; Hspa70; HST; inducible heat shock protein 70; Ischemia responsive 94 kDa protein; Spermatid-specific heat shock protein 70; Testis-specific heat shock protein-related; testis-specific heat shock protein-related gene hst70
基因别名: 70kDa; AI317151; APG-2; APG2; Hcp70.1; Hcp70.2; HEL-S-103; HEL-S-5a; HS24/P52; Hsc70t; Hsp110; hsp68; Hsp70; HSP70-1; HSP70-1A; HSP70-1B; HSP70-1L; HSP70-2; HSP70-3; HSP70-HOM; HSP70.1; HSP70.2; Hsp70.3; Hsp70A1; HSP70A2; HSP70I; Hsp70RY; HSP70T; HSP72; HSPA1; HSPA1A; HSPA1B; HSPA1L; HSPA2; HSPA4; HSPH2; Hspt70; Hst70; HSX70; hum70t; Irp94; mKIAA4025; Msh5; RY
virus receptor activity
G-protein coupled receptor binding
receptor binding
protein binding
ATP binding
ATPase activity
enzyme binding
heat shock protein binding
ubiquitin protein ligase binding
ATPase activity, coupled
histone deacetylase binding
protein binding involved in protein folding
unfolded protein binding
C3HC4-type RING finger domain binding
cadherin binding involved in cell-cell adhesion
glycolipid binding
protease binding
double-stranded RNA binding
poly(A) RNA binding
protein N-terminus binding
NF-kappaB binding
molecular_function
nucleotide binding
RNA polymerase II transcription factor binding
protein complex binding
activating transcription factor binding
response to unfolded protein
male meiosis
male meiosis I
spermatogenesis
spermatid development
response to heat
response to cold
positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle
protein refolding
synaptonemal complex disassembly
negative regulation of inclusion body assembly
positive regulation of calcium-transporting ATPase activity
positive regulation of gene expression
regulation of cell death
regulation of protein ubiquitination
negative regulation of protein ubiquitination
positive regulation of interleukin-8 production
cellular response to oxidative stress
cellular response to heat
ATP metabolic process
viral entry into host cell
protein stabilization
positive regulation of NF-kappaB transcription factor activity
negative regulation of cell death
cellular heat acclimation
positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway
regulation of cellular response to heat
positive regulation of tumor necrosis factor-mediated signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
binding of sperm to zona pellucida
positive regulation of protein targeting to mitochondrion
biological_process
telomere maintenance
DNA repair
protein folding
regulation of mRNA stability
cell-cell adhesion
negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of endoribonuclease activity
positive regulation of mRNA endonucleolytic cleavage involved in unfolded protein response
negative regulation of protein phosphorylation
negative regulation of gene expression
positive regulation of protein binding
negative regulation of apoptotic process
negative regulation of DNA binding
protein import into mitochondrial outer membrane
chaperone-mediated protein complex assembly
response to ischemia
mRNA catabolic process
negative regulation of cell proliferation
response to radiation
response to mechanical stimulus
neuron differentiation
negative regulation of cell growth
response to ethanol
positive regulation of erythrocyte differentiation
positive regulation of neuron differentiation
kidney development
positive regulation of angiogenesis
positive regulation of protein phosphorylation
multicellular organism development
positive regulation of T cell mediated cytotoxicity
defense response
negative regulation of myeloid cell apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of vasoconstriction
cell differentiation
