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Invitrogen
HSPA9 Polyclonal Antibody
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图: 1 / 6
HSPA9 Antibody (PA5-48035) in ICC/IF
Immunofluorescence analysis of HSPA9 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSPA9 Polyclonal Antibody (Product # PA5-48035) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11078) at a dilution... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-48035
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived recombinant human GRP75 Glu542-Gln679
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
0.2 mg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute at 0.2 mg/mL in sterile PBS.
靶标信息
The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75 and GRP78. These proteins serve a variety of roles. GRP75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP75 and GRP78 are unresponsive to heat stress and are induced by glucose deprivation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 75 kDa glucose-regulated protein; C3H-specific antigen; GRP 75; GRP-75; Heat shock 70 kDa protein 9; heat shock 70kDa protein 9A; Heat shock protein; heat shock protein 9A; heat shock protein cognate 74; Heat shock protein family A member 9; heat shock protein, 74 kDa, A; heat shock protein, A; HSP; MGC4500; Mortalin; MOT; mtHSP70; p66 MOT; PBP74; Peptide-binding protein 74; Stress-70 protein, mitochondrial
基因别名: 74kDa; Csa; GRP75; Hsc74; Hsp74; Hsp74a; HSPA9; Hspa9a; HSPA9B; Mortalin; Mot-2; Mot2; mt-HSP70; Mthsp70; Pbp74
UniProt ID: (Rat) P48721, (Mouse) P38647
Entrez Gene ID: (Rat) 291671, (Mouse) 15526
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