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Invitrogen
HSPA9 Polyclonal Antibody
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HSPA9 Antibody (PA5-79410) in ICC/IF
Immunofluorescence analysis of HSPA9 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HSPA9 Polyclonal Antibody (Product # PA5-79410) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Prod... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-79410
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human Grp75 (646-679aa KLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.01mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human A549 whole cell, human Jurkat whole cell, human HepG2 whole cell, rat testis tissue, rat PC-12 whole cell, mouse testis tissue, mouse NIH/3T3 whole cell. IHC: human lung cancer tissue, human intestinal cancer tissue. ICC/IF: A549 cell. Flow: HL-60 cell IP: HepG2 cell.
靶标信息
The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75 and GRP78. These proteins serve a variety of roles. GRP75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP75 and GRP78 are unresponsive to heat stress and are induced by glucose deprivation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 70 kda heat shock protein precursor; 75 kDa glucose-regulated protein; C3H-specific antigen; catecholamine regulated protein 40; catecholamine-regulated protein 40; CRP40; epididymis secretory sperm binding protein Li 124m; GRP 75; GRP-75; heat shock 70 kDa protein 9; heat shock 70kD protein 9B; heat shock 70kDa protein 9 (mortalin); heat shock 70kDa protein 9A; Heat shock protein; heat shock protein 9; heat shock protein 9A; heat shock protein cognate 74; heat shock protein family A member 9; heat shock protein, 74 kDa, A; heat shock protein, A; HSP; MGC4500; mortalin; mortalin, perinuclear; mortalin-2; mortalin2; mtHSP70; p66 MOT; p66-mortalin; peptide-binding protein 74; stress-70 protein, mitochondrial-like; unnamed protein product
基因别名: 74kDa; CRP40; CSA; EVPLS; GRP-75; GRP75; HEL-S-124m; Hsc74; Hsp74; Hsp74a; Hspa9a; HSPA9B; Mortalin; MOT; Mot-2; MOT2; Mthsp70; MTHSP75; PBP74; SAAN; SIDBA4
UniProt ID: (Human) P38646, (Mouse) P38647, (Rat) P48721
Entrez Gene ID: (Human) 3313, (Mouse) 15526, (Rat) 291671
protein folding
protein export from nucleus
response to toxic substance
iron-sulfur cluster assembly
erythrocyte differentiation
calcium ion transmembrane import into mitochondrion
protein refolding
positive regulation of apoptotic process
regulation of erythrocyte differentiation
negative regulation of erythrocyte differentiation
protein autophosphorylation
response to folic acid
cellular response to interleukin-1
response to thyroxine
negative regulation of hematopoietic stem cell differentiation
negative regulation of hemopoiesis
intracellular protein transport
inner mitochondrial membrane organization
protein import into mitochondrial matrix
negative regulation of apoptotic process
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