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Invitrogen
HSPB8 Monoclonal Antibody (7D8)
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HSPB8 Antibody (MA5-49225) in ICC/IF
Immunocytochemistry analysis of HSPB8 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in HSPB8 Monoclonal antibody (Product # MA5-49225) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for... View More
产品信息
MA5-49225
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
7D8
抗原
E.coli-derived human HSPB8/Hsp22 recombinant protein (Position: M1-T196).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Human HSPB8/Hsp22 shares 94.4% and 95.4% amino acid (aa) sequence identity with mouse and rat HSPB8/Hsp22, respectively.
Positive Control - WB: human Hela whole cell, human T-47D whole cell, rat RH35 whole cell. ICC/IF: A431 cell. Flow: CACO-2 cell, U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Alpha-crystallin C chain; crystallin, alpha C; DHMN 2; E2-induced gene 1 protein; heat shock 22kDa protein 8; heat shock 27kDa protein 8; Heat shock protein; heat shock protein 8; Heat shock protein beta-8; Heat shock protein family B member 8; HMN 2; HSB8; HSP; HSPB 8; HspB8; OTTHUMP00000239768; Protein kinase H11; Small stress protein-like protein HSP22
基因别名: CMT2L; CRYAC; DHMN2; E2IG1; H11; HMN2; HMN2A; HSP22; HSPB8; PP1629
UniProt ID: (Human) Q9UJY1, (Rat) Q9EPX0
Entrez Gene ID: (Human) 26353, (Rat) 113906
