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Agrisera
HY5/Protein long hypocotyl 5 Polyclonal Antibody
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HY5/Protein long hypocotyl 5 Antibody (AS12-1867) in WB
Samples: Marker used is Prestained Protein SHARPMASS™ VI Protein MW marker (5-245 k Da) from Euroclone. 1:40 µg of total protein from Arabidopsis thaliana wild type dark grown seedlings (negative control); 2:40 µg of total protein from Arabidopsis thaliana wild type light grown seedlings (positive control); 3:40 µg of total protein from Arabidopsis thaliana hy 5-215 light grown seedlings (negative control);... View More
产品信息
AS12-1867
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide, derived from Arabidopsis thaliana HY5 protein sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
HY5 encodes a nuclear basic leucine zipper (bZIP) transcription factor that functions as a central integrator of environmental light cues with developmental and physiological programs in Arabidopsis. The gene produces protein-coding transcripts whose product contains a conserved C-terminal bZIP DNA-binding/dimerization module (basic region plus leucine zipper) that mediates sequence-specific binding to ACGT-core light-responsive cis elements, particularly G-box motifs, thereby regulating transcription of downstream target promoters in response to illumination. Structural/biophysical work indicates HY5 is largely intrinsically disordered across its N-terminus, with conformational flexibility extending into the basic region, consistent with a regulatory protein that engages multiple partners and promoters. Functionally, HY5 acts predominantly as a positive regulator of photomorphogenesis, promoting light-dependent developmental transitions such as inhibition of hypocotyl elongation, seedling greening, and chloroplast development, and it contributes to stimulus-regulated root growth and patterning. HY5 abundance and activity are controlled post-translationally by light-dependent interaction with the E3 ubiquitin ligase COP1, which targets HY5 for proteasome-mediated degradation in darkness, enabling rapid tuning of light-responsive gene expression programs.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT5G11260; BZIP TRANSCRIPTION FACTOR HY5; BZIP56; ELONGATED HYPOCOTYL 5; F2I11.150; F2I11_150; REVERSAL OF THE DET PHENOTYPE 5; TED 5
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