Invitrogen

Heme oxygenase 2 Polyclonal Antibody

Name: Heme oxygenase 2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-143942

查看其他14个Heme oxygenase 2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (6)

Heme oxygenase 2 Antibody in Immunocytochemistry (ICC/IF)

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Heme oxygenase 2 Antibody (PA5-143942) in ICC/IF

Immunocytochemistry analysis of Heme oxygenase 2 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in Heme oxygenase 2 Polyclonal antibody (Product # PA5-143942) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 di... View More

Heme oxygenase 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Heme oxygenase 2 Antibody in Western Blot (WB)

Heme oxygenase 2 Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image Heme oxygenase 2 Polyclonal Antibody

产品信息

PA5-143942

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Heme oxygenase 2/HMOX2 recombinant protein (Position: H45-A301).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_3075156

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human Jurkat whole cell, human Hela whole cell, human K562 whole cellhuman PANC-1 whole cell. IHC: human lung squamous cell carcinoma tissue, human appendiceal adenocarcinoma tissue, human ovary tissue. ICC/IF: MCF-7 cell. Flow: MCF-7 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。